Please convert it to past tense and passive voice. Each group will be provided with two 20 g double-stranded DNA oligomers A and B in STE buffer (0.1M NaCl/ Tris/ 10 mM EDTA, pH 7.4). The sequence of the two oligomers used in this experiment is: 5’ GCATTGCGCAGGGCCGAG 3’ (GC rich) 3’ AATGGTACGTATACTTTAT3’ (AT rich) In this experiment, you are going to identity oligomer samples A and B, GC or AT rich, by UV spectrophotometric method. 1. Pipet 1 ml of each oligomer into a 1.5 ml Eppendorf tube and label the two tubes A and B. 2. The absorption wavelength is 260 nm. Use STE buffer provided to set blank. 3. You will be provided with two cuvettes. Use separate cuvette for each DNA sample. 4. Transfer 1 ml of DNA sample A to cuvette and measure the UV absorbance at 260 nm (A260) at room temperature. Repeat this step for Sample B. 5. Transfer the DNA back to the original Eppendorf tube, close it and heat it to 45C for 7 minutes. 6. Quickly transfer the sample from Eppendorf tube to cuvette, and measure the A260 as in step 3 immediately. 1 7. Repeat step 4 and 5 by heating the sample to 60C, 75C and 95C, and measure their A260. Before heating the sample at 75C and 95C, use a small piece of parafilm to wrap around the cap of the Eppendorf tube to prevent the cap from pop open. Slightly tap the tube if sample is condensed on the cap. 8. After 95C, cool down the tube to 75C, and 60C and then 45C for 7 minutes each, and measure the A260 at each temperature. 9. Do the heating and measuring A260 of Samples A and B in parallel to save time and maintain consistency.
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
Please convert it to past tense and passive voice.
Each group will be provided with two 20 g double-stranded DNA oligomers A and B in STE buffer (0.1M NaCl/ Tris/ 10 mM EDTA, pH 7.4). The sequence of the two oligomers used in this experiment is:
5’ GCATTGCGCAGGGCCGAG 3’ (GC rich) 3’ AATGGTACGTATACTTTAT3’ (AT rich)
In this experiment, you are going to identity oligomer samples A and B, GC or AT rich, by UV spectrophotometric method.
1. Pipet 1 ml of each oligomer into a 1.5 ml Eppendorf tube and label the two tubes A and B.
2. The absorption wavelength is 260 nm. Use STE buffer provided to set blank.
3. You will be provided with two cuvettes. Use separate cuvette for each DNA sample.
4. Transfer 1 ml of DNA sample A to cuvette and measure the UV absorbance at 260 nm (A260) at
room temperature. Repeat this step for Sample B.
5. Transfer the DNA back to the original Eppendorf tube, close it and heat it to 45C for 7 minutes.
6. Quickly transfer the sample from Eppendorf tube to cuvette, and measure the A260 as in step 3
immediately.
1
7. Repeat step 4 and 5 by heating the sample to 60C, 75C and 95C, and measure their A260. Before heating the sample at 75C and 95C, use a small piece of parafilm to wrap around the cap of the Eppendorf tube to prevent the cap from pop open. Slightly tap the tube if sample is condensed on the cap.
8. After 95C, cool down the tube to 75C, and 60C and then 45C for 7 minutes each, and measure the A260 at each temperature.
9. Do the heating and measuring A260 of Samples A and B in parallel to save time and maintain consistency.
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