Base pairs Data – all had Temp = 250C PH = 7 Binding Constant A & X [A] = 0.00373221M [X] = 0.00373221M [AX] = 0.0462678M 3.322 C & X [C] = 0.0469007M [X] = 0.0469007M [CX] = 0.00309935M 1.409 T & X [T] = 0.0452279M [X] = 0.0452279M [TX] = 0.00477212M 2.333 G & X [G] = 0.0469554M [X] = 0.0469554M [GX] = 0.00304456M
Question: Which base (A, C, T or G) corresponds to X in the unknown?
I did the experiment, got the data below, and calculated the binding constants. But I am TOTALLY lost as to how to figure this out! I don't even know what steps I would take.
|
Base pairs |
Data – all had Temp = 250C PH = 7 |
Binding Constant |
|
A & X |
[A] = 0.00373221M [X] = 0.00373221M [AX] = 0.0462678M |
3.322 |
|
C & X |
[C] = 0.0469007M [X] = 0.0469007M [CX] = 0.00309935M |
1.409 |
|
T & X |
[T] = 0.0452279M [X] = 0.0452279M [TX] = 0.00477212M |
2.333 |
|
G & X |
[G] = 0.0469554M [X] = 0.0469554M [GX] = 0.00304456M |
2.633 |
Step by step
Solved in 2 steps
What if all the data above was the same EXCEPT with the change of G & X? Their new data is below. Would base X still be T?
|
G & X |
[G] = 0.0447315M [X] = 0.0447315M [GX] = 0.0026845M |
1.3416 |
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