Phosphate buffered saline (PBS) is a very common reagent in cell biology labs that do cell culture since is it osmotically stable for most eukaryotic cells. It is often prepared as 10X PBS (10.5 mM KH2PO4, 1.552 M NaCl, 29.7 mM Na2HPO4) to save on shelf space. How would you prepare 1 L of 10X PBS given 1M KH2PO4, NaCl (FW 58.44), 906mM Na2HPO4?   What is the concentration of each component in 1X PBS? How would you prepare 750 ml of 1X PBS?

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