Oxaloacetate + ADP + Pi + H+ ---> Pyruvate + ATP + HCO3 AG°' = 13.91 kJ/mol Lactate + NAD+ ---> Pyruvate + NADH + H+ AGº 25.12 = kJ/mol Oxaloacetate + GTP --->> Phosphoenolpyruvate + GDP + CO₂ AG°= -28.40 kJ/mol HCO3 + H+ <--- CO2 + H2O AG°' = -41.45 kJ/mol kJ/mol GDP + ATP <--- <--- GTP + ADP AG°' = -0.003 Lactate + NAD++ 2ATP + H2O Phosphoenolpyruvate + NADH + 2ADP+ Pi + 3H+ From the above information, calculate the AGO for the overall reaction shown below the line, T = 37°C. Type your answer...

Introduction to General, Organic and Biochemistry
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Chapter23: Enzymes
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Problem 23.18P: 8 The optimal temperature for the action of lactate dehydrogenase is 36°C. It is irreversibly...
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Oxaloacetate  + ADP + Pi + H+      --->     Pyruvate + ATP + HCO3-                               ΔGo’ =  13.91  kJ/mol     

               Lactate + NAD+              --->       Pyruvate + NADH + H+                                     ΔGo’ =  25.12 kJ/mol               

             Oxaloacetate + GTP       --->            Phosphoenolpyruvate  + GDP + CO2           ΔGo’ = -28.40 kJ/mol

                          HCO3- + H+         --->         CO2 + H2O                                                       ΔGo’ =  -41.45  kJ/mol

                             GDP + ATP       --->         GTP + ADP                                                          ΔGo’ = -0.003  kJ/mol

_________________________________________________________________________________________________________________

Lactate + NAD+ + 2ATP + H2O    --->        Phosphoenolpyruvate + NADH + 2ADP + Pi + 3H+

                    From the above information, calculate theΔGo’ for the overall reaction shown below the line, T = 37oC.

Oxaloacetate + ADP + Pi + H+
--->
Pyruvate + ATP + HCO3
AG°' = 13.91
kJ/mol
Lactate + NAD+
--->
Pyruvate + NADH + H+
AGº 25.12
=
kJ/mol
Oxaloacetate + GTP
--->>
Phosphoenolpyruvate + GDP + CO₂
AG°=
-28.40 kJ/mol
HCO3 + H+
<---
CO2 + H2O
AG°' = -41.45
kJ/mol
kJ/mol
GDP + ATP
<---
<---
GTP + ADP
AG°' = -0.003
Lactate + NAD++ 2ATP + H2O
Phosphoenolpyruvate + NADH + 2ADP+ Pi + 3H+
From the above information, calculate the AGO for the overall reaction shown
below the line, T = 37°C.
Type your answer...
Transcribed Image Text:Oxaloacetate + ADP + Pi + H+ ---> Pyruvate + ATP + HCO3 AG°' = 13.91 kJ/mol Lactate + NAD+ ---> Pyruvate + NADH + H+ AGº 25.12 = kJ/mol Oxaloacetate + GTP --->> Phosphoenolpyruvate + GDP + CO₂ AG°= -28.40 kJ/mol HCO3 + H+ <--- CO2 + H2O AG°' = -41.45 kJ/mol kJ/mol GDP + ATP <--- <--- GTP + ADP AG°' = -0.003 Lactate + NAD++ 2ATP + H2O Phosphoenolpyruvate + NADH + 2ADP+ Pi + 3H+ From the above information, calculate the AGO for the overall reaction shown below the line, T = 37°C. Type your answer...
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