Outline 5 factors to be considered when performing a similarity search with a short peptide/nucleotide sequence.

Q: What does BLAST stand for? Basic Local Alignment Search Tool Basic Local Alignment SequenceTool…

A: NCBI is the database that stores the information regarding the gene sequences and protein sequences.…

Q: alignment in genomic research, list types of alignments and corresponding errors associated with…

A: Introduction :- Alignment is a fundamental technique used in genomic research to compare and match…

Q: pMdawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI:…

A: Plasmids are double-stranded circular DNA found in bacteria. These contain genes related to…

Q: Explain Peptide Mapping and Internal Sequencing of Proteins from Acrylamide Gels.

A: Peptide mapping is an identity test for proteins, those obtained by rDNA technology. It involves the…

Q: Give any 3 rules to follow when designing primers

A: DNA synthesis is carried out by DNA polymerase. In addition to the template, the DNA polymerase also…

Q: This descriptor refers to the highest alignment score of all matched sequences to the sequence…

A: The highest alignment score is determined by the BLAST algorithm and is based on the number of…

Q: A very common molecular biology research method is to analyze cell or tissue homogenates by…

A: In molecular biology research, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting,…

Q: Which of the following sequences is less favorable to find in a folded beta? Select one:…

A: Two types of secondary structures are abundant in protein: alpha helix and beta sheets.  The alpha…

Q: You have an aqueous solution containing: Alaine (a mono-amino, monocarboxylic acid) Fructose (a…

A: An assay can be defined as an analytic procedure that is used in laboratory medicine, mining for…

Q: EcoRI recognizes G A-A-T-T-C sequence and cleave/ cut between G and A. How will the DNA fragments…

A: A restriction site or recognition site, is a specific sequence of nucleotides in DNA that a…

Q: Representations of sequencing chromatograms for variants of the a chain of human hemoglobin are…

A: The sequencig chromatograms given in question are generated as we conduct Automated DNA sequencing…

Q: Explain the difference between sequencing by chain termination and sequencing by synthesis. Which…

A: Chain termination process employs dideoxynucleoside triphosphates (ddNTPs) lacking OH (hydroxyl)…

Q: What is the methodology in RNA Biology | Taylor & Francis Online

A: The question asks about the methodology used in RNA Biology. It wants to know the techniques and…

Q: In the following gel showing stained bands of the Alu insertion sequence, what is the gem of…

A: In the gel, the presence of a band at 941 bp indicates the presence of the Alu insertion sequence,…

Q: why is gap opening penalized more than gap extension in doing alignment of sequence similarity?

A: Gap penalties should be use in sequence alignment to obtain the best outcome between the two…

Q: A portion of the coding sequence of a cloned gene is shown…

A: The process of converting a DNA strand into mRNA is known as Transcription. The strand having the…

Q: See the attachment and answer the following parts of the question: A) If the binturong genome is…

A: Binturong is a eukaryote in which there is highly repetitive DNA sequence is present. The copies of…

Q: Yu raach aitantin a ab tht studies mucleic acids. Your advisor gave you four tubes for analysis.…

A: Each organism have a genetic meterial by which they regulate, inherited and constant their…

Q: Discuss the principles , uses, advantages and disadvantages of illumina sequencing method

A: DNA copies obtained from PCR is subjected for gene sequencing through different gene sequencing…

Q: Although techniques are available for determining the sequences of amino acids in proteins, it is…

A: It is easier to sequence proteins indirectly by determining the base sequence of the gene for…

Q: When comparing (i.e., aligning) two or more genetic sequences, itis sometimes necessary to put in…

A: Introduction Sequence alignment is necessary to compare sequences. This can be used to find the…

Q: * Part of a sequence of DNA from a person without this genetic disease is: TAG TAA AAA CCA CCC AGG…

A: Anticodons are nucleotide sequences that are the opposite of codons. They're located in tRNAs, and…

Q: Ruwaifa want to compares a nucleotide query sequence what option he will opt in BLAST and why?

A: BLAST is Basic local alignment search tool used to compare the queries in a DNA database. They are…

Q: Please explain how to use the global alignment algorithm: Needleman-Wunsch. Use the match 3,…

A: Given:Protein sequence 1: PHWGProtein sequence 2: HWEGMatch score: 3Mismatch score: -3Gap penalty:…

Q: 1. What nitrogenous base is found in position Y? 2. What nitrogenous base is found in position X? 3.…

A: Nucleotides are the building blocks of DNA and RNA. The process of transcription produces messenger…

Q: Discuss why it is useful to search a database to identify sequencesthat are homologous to a newly…

A: The most basic operations in bioinformatics involves searching for similarities, or homologies,…

Q: Hi, I would like to know which program is used for the graphical presentation of the results of a…

A: A genome wide linkage association study (GWAS) is an approach used in genetic research to associate…

Q: Describe the various characteristics of the Watson–Crick doublehelix model for DNA.

A: The hereditary ingredient in the cell is genetic material. It contains all of an organism's…

Q: write down the applications of pair wise and multipal sequence alignment in the field of…

A: Multiple sequence alignment or MSA of nucleic acids i.e. DNA, RNA and proteins is nowadays one of…

Q: 1. You are investigating a protein that has the amino acid sequence N... Ala – Thr - Asn – Trp – Lys…

A: BASIC INFORMATION  GENETIC CODE  The codes are written in a group of three thus it is triplet.…

Q: The best molecular technique to quantify the gene transcripts is (write in fu!!).

A: The Central Dogma concept states that "DNA makes RNA and RNA makes protein". This concept is…

Q: Explain the Molecular Techniques for Analyzing RNA ?

A: A gene is the essential physical and functional unit of heredity. They are comprised of DNA…

Q: Southern blotting is a method used in molecular biology for detection of a specific DNA sequence in…

A: Nucleic acid blotting is a well-established technique for locating a gene or sequence of interest…

Q: Describe the various characteristics of the Watson–Crick doublehelix model for DNA.

A: DNA (deoxyribonucleic acid) is the nucleic acid present in the cells of living beings. It is mostly…

Q: Summarize the basic steps in Differential Expression analysis starting with a directory of…

A: We need to summarize the basic steps in Differential Expression analysis starting with a directory…

Q: What are the similarities and/or differences between interpreting Multiple Sequence Alignment (MSA)…

A: Multiple sequence alignment (MSA) is the process or outcome of aligning three or more biological…

Q: 3. After aligning four protein sequences, following matrix was derived. Sequence Name the Seq-1…

A: DNA and RNA are the nucleic acids found in all living organisms. DNA and RNA are made up of…