ou want to clone the yeast ABC gene into an expression vector. Is it possible to induce the rotein expression of a yeast gene from prokaryotic expression vector that is transformed into scherichia coli (E. coliy? Explain your reason.
Q: Describe the difference between a transcriptional fusion and a translational reporter gene fusion.…
A: Transcription fusion means cloning the promoter of interest, upstream of any transcription unit,…
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A: NOTE:- As you have posted multiple questions under one, we will solve the first part for you, to…
Q: . You are analyzing two different cells that transcribe the same gene called neurexin (NRXN). The…
A: Hi dear, here's your answer.
Q: Explain why the glucocorticoid receptor binds next to the core promoter of some genes, but not next…
A: Gene expression is a process in which the information present in the particular sequences is…
Q: How would a mutation which prevented Gal3 from binding to Gal80 affect gene expression from the GAL…
A: Galactose metabolizing genes express themselves in the presence of galactose. Galactose is…
Q: Geneticists isolated six trains of E. coli (strains 1-6) whose lac operons had one of the following…
A: LAC operon of E. coli is an example of inducible gene expression system. Lactose acts as inducer…
Q: Which of the following incorrectly describes the difference between eukaryotic and prokaryotic gene…
A: Prokaryotic organisms are single-celled creatures that lack the cell nucleus, and their DNA…
Q: Many eukaryotic promoter regions contain CAAT boxes with consensus sequences CAAT or CCAAT…
A: The CAAT box is popularly known as a core promoter or basal promoter or simply the Promoter. It is…
Q: PROTEIN X HAS THE POTENTIAL FOR MEDICAL APPLICATION IN HUMAN. IT IS ORIGINALLY FROM A PLANT AND HAD…
A: Protein expression is an element of DNA Recombinant innovation or hereditary designing. Protein…
Q: name TWO mechanisms of control of gene expression that are only used by eukaryotes and discuss why…
A: The majority of gene expression is regulated at the transcriptional level, owing to protein binding…
Q: A series of exonuclease deletions were used to study the promoter of the rice hemA gene, giving the…
A: A promoter can be defined as a DNA sequence where proteins bind and initiate transcription of an…
Q: When wild-type E. coli are grown in media with high lactose and no glucose, which of the following…
A: The lactose operon (lac operon) is an operon required for the transport and metabolism of lactose in…
Q: ith examples explain the importance of Non-coding ribonucleic acid interference in regards to gene…
A: A DNA sequence's genes are translated into amino acid sequences during the process of gene…
Q: You made four mutants for a promoter sequence in DNA and studied them for transcription. The results…
A: Promoter are the sequence present in the DNA. These allow the binding of transcription factor and…
Q: A lac operon containing one mutation was cloned into a plasmid, which was introduced by…
A: (a) The plasma i gene forms such a type of repressor that will not be able to bind the inducer.
Q: Eukaryotes have a multitude of ways of regulating gene expression. Why are all these regulatory…
A: gene regulation is the process used to control the timing, location and amount in which genes are…
Q: Some eukaryotic mRNAs have an AU-rich element in the 3′ untranslated region. What would be the…
A: The 3’ UTR is the untranslated region in the mRNA that follows the termination codon of translation.…
Q: he lac repressor and the pur repressor are homologous proteins with very similar three-dimensional…
A: Purine repressor (PurR) is a DNA-binding protein that is involved in the transcription process. Its…
Q: Consider a mutation in -10 BOX promoter consensus sequence (TATAAT) in the prokaryote recA gene…
A: TATTAT is called pribnow box in prokaryotes and is a promoter sequence that tells the Enzymes where…
Q: What is the significance of reverse transcriptase inthe cloning of animal genes for expression in…
A: The enzyme reverse transcriptase plays an important role in generating copy of the translated mRNA…
Q: The following statement occurs early in this chapter:“. . . a crucial step in the regulation of many…
A: Transcription is a process in which a sequence of DNA is transcribed into mRNA. In bacterial…
Q: Explain how the data shown indicate that two operator sites are necessary for repression of the lac…
A: Introduction: Lac operon exerts positive control on catabolite repression that leads to an increase…
Q: Upon identification of the DNA regulatory sequence responsible for translating a given gene, you…
A: The regulatory proteins binding along with enhancer sequence will result in a shift that occurs in…
Q: The PIC is highly conserved among species, from yeast to humans. Why is the positioning of the first…
A: PIC stands for the pre-initiation complex. The core promoter region comprising of the initiator…
Q: Suppose you have six strains of E. coli. One is wildtype, and each of the other five has a single…
A: Escherichia coli (E.coli) has the following five mutant strains. Lac Z-, lacy-, lacl-, Oc, and…
Q: When disrupting a mouse gene by knockout, why is it desirable to breed mice until offspring…
A: In knockout animal, a targeting vector having a gene of interest is introduced into the cells. The…
Q: Cloning of a eukaryotic gene can be carried out in Escherichia coli. However, for the protein…
A: Introduction Eukaryotes are organisms having well-defined cellular organelles that are specified to…
Q: Bacterial DNA containing an operon encoding three enzymes is introduced into chromosomal DNA in…
A: Prokaryotes are the organisms that lack the cell nucleus and membrane-bound organelles. They have…
Q: Some vectors used in cloning experiments contain bacterial promotersthat are adjacent to unique…
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: Gene-specific transcription factors interact with DNA to control gene expression. At which surface…
A: Transcription is the process of copying genetic information from one strand of DNA into RNA. The…
Q: From the list given - choose all the regulatory sequences that you think would control the…
A: A regulatory sequence is a nucleic acid molecule segment that has the ability to increase or…
Q: Explain why RNAi would be a less effi cient mechanism for regulating the expression of specifi c…
A: RNA interference (RNAi) is a biological process in which RNA molecules inhibits gene expressions or…
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A: krox20 is also known as early growth response protein 2, it is a transcription regulatory factor.it…
Q: Explain how the following mutations would affect transcription of the yeast GAL1 gene in the…
A: The mutation is defined as the change in a sequence of DNA. It will occur when a gene of DNA is…
Q: A graduate student studying the pathogenic bacteria Acinetobacter baumannii made cDNA from…
A: Biotechnology is the use of our understanding of biological processes to develop useful applications…
Q: Does transcriptional reporter with GFP allow you to see the expression of the different isoforms?…
A: Alternative splicing (AS) plays a fundamental role in the diversification of protein function and…
Q: Why is it adaptive for a bacterium to not express the genes that encode for that lactose utilization…
A: Lac operon or the lactose operon is a component system containing genres that are important for the…
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A: Recombinant DNA technology or RDT is a biotechnological technique that is used to take a gene of…
Q: For the ovalbumin gene shown, indicate the locations of the following: (a) transcription start site,…
A: The transcription start site is the site on the DNA from which the first RNA nucleotide (+1) is…
Q: Is it possible to induce the protein expression of a yeast gene from a prokaryotic expression vector…
A: Yes this is possible... First we isolate the yeast species which are needed for protein expression.…
Q: Both eukaryotic and prokaryotic promoters O contain enhancer or silencer sequences. O contain TATA…
A: A promoter is a region of DNA where transcription of a gene is initiated. Promoters are a vital…
Q: . All mutations that abolish function of the Rho termination protein in E. coli are conditional…
A: Rho factors are the type of proteins present in prokaryotes that are responsible for transcription…
Q: The yeast gene SER3, whose product has a role in serine biosynthesis, is repressed during growth in…
A: The yeast cell consists of a cell wall and a plasma membrane. The cell wall is composed of mostly…
Q: Which of the following parts of the eukaryotic promoter are bound by general transcription factors?…
A: As visible In image GTF general transcription factor bind to core protein , proximal elements
Q: You want to clone a eukaryotic gene and express the corresponding protein in yeast. However, the…
A: Most eukaryotic genes contain segments of coding sequences (exons) interrupted by noncoding…
Q: The APETALA1:GUS line you were given is a transcriptional fusion. Given that APETALA1 encodes a…
A: Introduction : Transcriptional reporters is a promoter fragment from gene of interest harboring GFP.…
Q: however, a transversion occurred which changed the G nucleotide to T, what outcome(s) are likely?…
A: Promoter Is it a sequence of DNA in up stream or near the beginning of the gene. It regulates the…
Q: You have isolated two different mutants (reg1 andreg2) causing constitutive expression of the emu…
A: A constitutive mutation refers to the mutation in which the bacterial strains having mutation…
Q: Many promoter regions contain CAAT boxes containing consensus sequences CAAT or CCAAT approximately…
A: There are many different non-coding sequences in the DNA (deoxyribonucleic acid) that help in…
Q: In the laboratory, you want to study protein that is normally toxic to E. coli cells. You wish to…
A: AraBAD promotor is a structural gene of L-arabinose operon in E.coli. AraBAD basically are 3…
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protein expression of a yeast gene from prokaryotic expression vector that is transformed into
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- Termicin is a small antifungal protein in termites that is produced by cells and secreted into termite saliva in response to a pathogen. In vitro translation of the termicin-encoding gene is performed, and the effects of that product are compared to those of termicin extracted from a termite. You see that extracted termicin exhibits more antifungal behavior than in vitro translated termicin. After further analysis, you see that extracted termicin contains 3 disulfide bonds, while in vitro translated termicin contains zero. The addition of microsomes to the in vitro translation reaction results in termicin with all 3 disulfide bonds. What experimental condition is most likely responsible for this difference? A. in vitro translation was not performed at the correct temperature affecting protein folding B. a mutation occurred during in vitro translation, leading to differences in disulfide bond formation O C. the UPR can not be activated in vitro, therefore, this protein can only be…Describe how transcription would be affected in the Galactose metabolizing pathway in Yeast in the presence of the following mutations. 1. A mutation that resulted in an inability of Gal80 to enter the nucleus. 2. A mutation that resulted in a lack of ability of Gal3 to bind galactose.name TWO mechanisms of control of gene expression that are only used by eukaryotes and discuss why each of those mechanisms wouldn’t be possible in prokaryotes.
- The streptolysin S toxin made by S. pyogenes is encoded by a 9-gene operon, sagABCDEFGHI. Thinking about what a 3-line diagram would look like for this operon, answer the following questions. Write numeric answers only. For example, if your answer is 6 promoters, write only 6. 1) How many promoters control the expression of these genes? 2) How many locations does RNA Polymerase bind to get full expression of these genes? 3) How many ribosome binding sites are needed for full protein expression? 4) How many start codons will be needed for full protein expression? 5) How many mRNA strands will be produced with full operon expression? 6) How many proteins will be produced with full protein expression? 1In the laboratory, you want to study protein that is normally toxic to E. coli cells. You wish to grow this protein in E. coli and purify it from E. coli. Your advisor suggests cloning the gene into an expression vector that uses the araBAD promoter. Explain why is it ideal to use the araBAD promoter for expression of your gene of interest in E. coli cells?You have isolated two different mutants (reg1 and reg2) causing constitutive expression of the emu operon (emu1 emu2). One mutant contains a defect in a DNA-binding site, and the other has a loss-of-function defect in the gene encoding a protein that binds to the site. Is the DNA-binding protein a positive or negative regulator of gene expression? Explain. To determine which mutant has a defect in the site and which one has a mutation in the binding protein, you decide to do an analysis using F′ plasmids. Assuming you can assay levels of the Emu1 and Emu2 proteins, what results do you predict for the two strains (i and ii; see descriptions below) if reg2 encodes the regulatory protein and reg1 is the regulatory site? Explain. F′ (reg1− reg2+ emu1− emu2+)/reg1+ reg2+ emu1+ emu2− F′ (reg1+ reg2− emu1− emu2+)/reg1+ reg2+ emu1+ emu2−
- The DNA sequence of the promoter region of E. coli xyzA gene is shown below. Transcription start site is the A (in bold) at position 43. 5 10 15 20 25 30 35 40 45 50 GAGCT GTTGA CAATT AATCA TCGAA CTAGT TAACT AGTAC GCAAG TTCAC Mutations were introduced in the sequence to identify residues important for gene expression. Indicate the effect of the following mutations on xyzA expression (increase, decrease, no effect, cannot be predicted). Provide reasoning for each answer. A. G3A (G at position 3 was changed to A) G9A Deletion of TCA at position 18-20 C22A T31A, A32T double mutant T35G G45C C48A B. What are the promoter sequences of the gene?A strain of Arabidopsis thaliana possesses a mutation in the APETALA2 gene. As a result of this mutation, much of the 3′ UTR of the mRNA transcribed from the gene is deleted. What is the most likely effect of this mutation on the expression of the APETALA2 gene?You have isolated different mutants (reg1 and reg2) causing constitutive expression of the emu operon (which has genes emu1 and emu2). One mutant contains a defect in a DNA-binding site, and the other has a loss-of-function defect in the gene encoding a protein that binds to the site. Is the DNA-binding protein a positive or negative regulator of gene expression?
- A number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the lowest beta-galactosidase enzyme activity, when grown in the lactose medium. Orpt o* z* r* Orpt ot z* Y OrptoztY Orrotzr OrPotz*YIn the galactose operon of Escherichia coli, a repressor, encoded by the galR gene, binds to an operator site, galo, to regulate the expression of three structural genes, galE, galT, and galK. Expression is induced by the presence of galactose in the media. For each of the strains listed, would the cell show constitutive, inducible, or no expression of each of the structural genes? (Assume that galR−is a loss-of-function mutation.) galR− galo+ galE+ galT+ galK+ galR+ galoc galE+ galT+ galK+ galR− galo+ galE+ galT+ galK−/ galR+ galo+ galE− galT+ galK+ galR− galoc galE+ galT+ galK−/ galR+ galo+ galE− galT+ galK+The locations of the TATA box in two species of yeast, Saccharomyces pombe and Saccharomyces cerevisiae, differ dramatically. The TATA box of S. pombe is about 30 nucleotides upstream of the transcription start site, similar to the location in most other eukaryotic cells. However, the TATA box of S. cerevisiae is 40 to 120 nucleotides upstream of the start site. To better understand what sets the start site in these organisms, researchers at Stanford University conducted a series of experiments to determine which components of the transcription apparatus of these two species could be interchanged (Y. Li et al. 1994. Science 263:805–807). In these experiments, different general transcription factors and RNA polymerases were switched in S. pombe and S. cerevisiae, and the effects of each switch on the level of RNA synthesis and on the starting point of transcription were observed. The results from one set of experiments are shown in the table below. Components cTFIIB, cTFIIE, cTFIIF,…
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