One thing we've mentioned a lot in this class is that pyruvate can only undergo further oxidation (via PDH enzyme and TCA cycle), if there is sufficient O2 available. But why exactly is this the case? Is O2 needed specifically in one of the reactions of the TCA cycle? if so, which TCA cycle reaction is it needed for and what is its role in that reaction? If you do not think that O2 is specifically needed in a reaction of the TCA cycle, explain why it is needed for the TCA cycle to function?

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