Part A: Write the balanced reaction catalyzed by phophoenolpyruvate carboxylase (PPC). Remember to add any cofactors, and to draw the structure formulas (a line-bond representation is fine) for the main substrate and product of the reaction. Hint: For this one, look on Wikipedia to get all the cofactors. The PPC in the Wikipedia article takes PEP and makes it into oxaloacetate without the use of a ribonucleotide at all. So, just have Pi as a product. Part B: Draw a diagram (flow chart) that describes how E. coli obtains oxaloacetate via the glyoxylate cycle when acetate (in the form of acetyl-CoA), not glucose, is provided as a food source. Note in the circle, the bit of the glyoxylate cycle which makes acetyl-CoA into oxaloacetate in the fewest number of steps. Part C: What does oxaloacetate lead to in the gluconeogenesis pathway?
Electron Transport Chain
The electron transport chain, also known as the electron transport system, is a group of proteins that transfer electrons through a membrane within mitochondria to create a gradient of protons that drives adenosine triphosphate (ATP)synthesis. The cell uses ATP as an energy source for metabolic processes and cellular functions. ETC involves series of reactions that convert redox energy from NADH (nicotinamide adenine dinucleotide (NAD) + hydrogen (H)) and FADH2(flavin adenine dinucleotide (FAD)) oxidation into proton-motive force(PMF), which is then used to synthesize ATP through conformational changes in the ATP synthase complex, a process known as oxidative phosphorylation.
Metabolism
Picture a campfire. It keeps the body warm on a cold night and provides light. To ensure that the fire keeps burning, fuel needs to be added(pieces of wood in this case). When a small piece is added, the fire burns bright for a bit and then dies down unless more wood is added. But, if too many pieces are placed at a time, the fire escalates and burns for a longer time, without actually burning away all the pieces that have been added. Many of them, especially the larger chunks or damp pieces, remain unburnt.
Cellular Respiration
Cellular respiration is the cellular process involved in the generation of adenosine triphosphate (ATP) molecules from the organic nutritional source obtained from the diet. It is a universal process observed in all types of life forms. The glucose (chemical formula C6H12O6) molecules are the preferred raw material for cell respiration as it possesses a simple structure and is highly efficient in nature.
The enzyme pyruvate carboxylase is discovered in a bacterium that was thought not to contain it; in this case study, you'll see how researchers study and characterize the enzyme, and, ultimately, show how it fits into a
Part A: Write the balanced reaction catalyzed by phophoenolpyruvate carboxylase (PPC). Remember to add any cofactors, and to draw the structure formulas (a line-bond representation is fine) for the main substrate and product of the reaction. Hint: For this one, look on Wikipedia to get all the cofactors. The PPC in the Wikipedia article takes PEP and makes it into oxaloacetate without the use of a ribonucleotide at all. So, just have Pi as a product.
Part B: Draw a diagram (flow chart) that describes how E. coli obtains oxaloacetate via the glyoxylate cycle when acetate (in the form of acetyl-CoA), not glucose, is provided as a food source. Note in the circle, the bit of the glyoxylate cycle which makes acetyl-CoA into oxaloacetate in the fewest number of steps.
Part C: What does oxaloacetate lead to in the gluconeogenesis pathway?
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