(B) 28 S 1 2 3 4 5 6 7 8 9 10 11 18 S 15% 28 S C 60% 18 S 15% 60% Sucrose S Questions 1. What molecules are detected by UV absorption at the top of the gradient (fraction 1)? 2. What complexes are detected by UV absorption in fraction 3? 3. What complexes are detected by UV absorption in fraction 5? 4. What complexes are detected by UV absorption in fractions 6-11? How does starvation affect the sedimentograms? How does it affect translation? Explain your conclusions. 6. Which step of translation (initiation or elongation) is mainly affected by starvation? OD₂ 260 1 2 3 (A) Control Starvation 4 5 6 7 8 9 10 11 Fraction
(B) 28 S 1 2 3 4 5 6 7 8 9 10 11 18 S 15% 28 S C 60% 18 S 15% 60% Sucrose S Questions 1. What molecules are detected by UV absorption at the top of the gradient (fraction 1)? 2. What complexes are detected by UV absorption in fraction 3? 3. What complexes are detected by UV absorption in fraction 5? 4. What complexes are detected by UV absorption in fractions 6-11? How does starvation affect the sedimentograms? How does it affect translation? Explain your conclusions. 6. Which step of translation (initiation or elongation) is mainly affected by starvation? OD₂ 260 1 2 3 (A) Control Starvation 4 5 6 7 8 9 10 11 Fraction
Chapter17: Drugs And Dissection
Section: Chapter Questions
Problem 3WS
Related questions
Question
Experiment The effect of starvation on protein synthesis in Caenorhabditis elegans was studied in this experiment. Worms were kept well fed (C, control) or starved (S) for 6 hours. Ribosomal fractions were isolated from the two groups and centrifuged in separate tubes through 15-60% sucrose density gradients. Fractions were collected from the top of the sucrose gradient. UV absorption at 260 nm (OD260) was measured (A), and RNA isolated from each fraction was analyzed by agarose gel electrophoresis (B).
Transcribed Image Text:(B)
28 S
1 2 3 4 5 6 7 8 9 10 11
18 S
15%
28 S
C
60%
18 S 15%
60%
Sucrose
S
Questions
1. What molecules are detected by UV absorption at the top of the gradient (fraction 1)?
2. What complexes are detected by UV absorption in fraction 3?
3. What complexes are detected by UV absorption in fraction 5?
4. What complexes are detected by UV absorption in fractions 6-11?
How does starvation affect the sedimentograms? How does it affect translation? Explain your
conclusions.
6. Which step of translation (initiation or elongation) is mainly affected by starvation?
Transcribed Image Text:OD₂
260
1 2 3
(A)
Control
Starvation
4 5 6 7 8 9 10 11
Fraction
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps
Recommended textbooks for you
Case Studies In Health Information Management
Biology
ISBN:
9781337676908
Author:
SCHNERING
Publisher:
Cengage
Essentials of Pharmacology for Health Professions
Nursing
ISBN:
9781305441620
Author:
WOODROW
Publisher:
Cengage
Case Studies In Health Information Management
Biology
ISBN:
9781337676908
Author:
SCHNERING
Publisher:
Cengage
Essentials of Pharmacology for Health Professions
Nursing
ISBN:
9781305441620
Author:
WOODROW
Publisher:
Cengage
Medical Terminology for Health Professions, Spira…
Health & Nutrition
ISBN:
9781305634350
Author:
Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder
Publisher:
Cengage Learning