A mouse fibroblast cell line in culture was treated with a compound derived from a fungus, which caused the cells to stop dividing. In order to understand how this compound inhibits cell division, a culture of these cells was grown and treated with the compound, and cell samples were taken at increasing times after addition of the compound. The cells in each sample were stained with a fluorescent DNA-binding dye and analyzed by flow cytometry. Data are shown below for four cell samples taken at 0, 5, 16, and 24 hours after addition of the compound. Fluorescence intensity is shown on the x-axis and cell number on the y-axis. Cell number Oh 24 h التالي 200 16 h 5 h I 100 200 250 0 100 200 250 0 100 200 200 250 0 100 200 250 Fluorescence In 2-3 sentences, explain which cell cycle phase is blocked by the compound, and how you came to that conclusion.

A mouse fibroblast cell line in culture was treated with a compound derived from a fungus, which caused the cells to stop dividing. In order to understand how this compound inhibits cell division, a culture of these cells was grown and treated with the compound, and cell samples were taken at increasing times after addition of the compound. The cells in each sample were stained with a fluorescent DNA-binding dye and analyzed by flow cytometry. Data are shown below for four cell samples taken at 0, 5, 16, and 24 hours after addition of the compound. Fluorescence intensity is shown on the x-axis and cell number on the y-axis. Cell number Oh 24 h التالي 200 16 h 5 h I 100 200 250 0 100 200 250 0 100 200 200 250 0 100 200 250 Fluorescence In 2-3 sentences, explain which cell cycle phase is blocked by the compound, and how you came to that conclusion.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter10: Cell Division And Mitosis

Section: Chapter Questions

Problem 1ITD: Biologists have long been interested in the effects of radiation on cells. In one experiment,...

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In order to investigate the cell cycle in HEK293 cells in culture, I ran a flow cytometry experiment. I labeled their nuclei with DAPI to analyze DNA content. In these flow cytometry plots below, label the two main phases of the cell cycle that you can see. Briefly, explain your answer.
For most cell types, serum must be added to the media to get cells to grow and proliferate in culture. Nevertheless, serum is very expensive and can complicate experiments utilizing the cells. Many companies have developed "artificial serum" which enables cells to multiply without the use of serum. See data below. What are likely components of the artificial serum that enables cells to grow. Explain (limit 3-4sentences). Non 2% Human plasma 1% Artificial serum(XF) 1% Artificial serum(AF) 104 cells/cm² Cell density cultured after 4 days 127 10- 2 0 NON 2% 1% 1% Human Artificial Artificial plasma serum(XF) serum(AF) PDL [times] 30- 25+ 20- 15- 10- 5- 0 -2% Human plasma 0 Subculture 1% Artificial serum (XF) 1% Artificial serum(AF) 5 10 15 20 25 30 Culture days Medium: HFDM-1 (+) was used.
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Hoechst 33342 is a membrane-permeant dye thatfluoresces when it binds to DNA. When a population ofcells is incubated briefly with Hoechst dye and then sortedin a flow cytometer, which measures the fluorescence ofeach cell, the cells display various levels of fluorescence asshown in Figure Q17–1.
You treat some cancer cells with a new therapeutic that you hope will kill them. You run an MTT assay on the results and notice that the sample from the control cells (not treated with drug) turns purple upon addition of the MTT and the sample from the cells treated your drug are clear after adding MTT. As you walk down the hall to run your sample on the spectrophotometer, how are you feeling? (a) frustrated, (b) unsure, (c) excited. I understand that if the cell are alive, they will have functioning mitochondria, the mitochondria will be producing NADH and NADPH which will allow some enzymes in the cell to convert empty T which is colorless, into a colored form as and die. The colorelss means less dye with present, which means the less empty T was converted, meaning that we had less mitochondrial activity, which means we had less live cells. I think that the answer should be (a) frustrated. I am not sure if I am right. Glad if the expert advise.
Anticancer drugs that affect microtubules are often used in cancer treatment. Explain why this is the case and the detailed mechanism(s) of how these drugs inhibit microtubules that make them effective.
Three actively growing cultures of the same strain of yeast (saccharomyces cerevisiae) are used. Culture A has been incubated for 48 hours and Culture B has been incubated for 24hours and Culture C for 6 hours. a) Make a drawing of the cells from each culture to identify the various stages of the cell division. b) Is there any difference between the 3 cultures?
A common step in the isolation of cells from asample of animal tissue is to treat the tissue with trypsin,collagenase, and EDTA. Why is such a treatment nece-ssary, and what does each component accomplish? Andwhy does this treatment not kill the cells?
Suppose cells in an experiment had been labeled with green fluorescent tubulin. At the onset of Anaphase B, you use your laser to bleach a stripe across all of the microtubules on one side of the spindle as shown by the dashed line. This does not hurt the function of the microtubules in any way, but the bleached, nonfluorescent subunits in the microtubules now serve to mark a fixed location relative to the (+) and (-) ends. pl. membr. spindle A. Label one of each of the following: kinetochore MT, astral MT, polar MT. Indicate (+) and (-) ends. B. As the cell progresses through anaphase B, do the bleached spots get closer to, further from, or stay the same distance from the spindle pole they are embedded in? Why? Do they get closer to, further from, or stay the same distance from the plasma membrane? Why?
12. The figure shows a micrograph of colchicine treated onion root tip cells prepared using squash preparation by Alex during practical. Name the stage of cell cycle of the cell pointed by arrow head in the micrograph. A) Prophase B) Metaphase C) Anaphase O D) Telophase
Which protein or proteins mediate(s) the nucleation of microtubules? Arp 4 O Formin OY-TURC O Arp 2/3 < Previous
Consider you are growing HEK293 cells in the lab. After you subculture the cells several times, you realize that proliferation efficiency is decreased although morphology of the cells is not changed. What may be the problem? How can we troubleshoot?