Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?
Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?
Chapter2: Aquatic Plants And Animals
Section: Chapter Questions
Problem 2KA
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Transcribed Image Text:Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10'
cells/ml and are asked to carry out the sample dilution process shown in the figure below.
100μl 100μl 100μl
100μl 100μl
0.9ml 0.9ml 0.9ml
H2O H₂O
6.9ml
0.9ml
H₂O
H₂O
H₂O
Original 10-1 102 10-3 104
Culture
105
100μl
100μl
100μl
Plate A
Plate B
Plate C
a.
How many colonies should have been present on Plate A in this example? - Answers must be
whole numbers as partial colonies are not expected.
b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you
do not know the concentration of the original culture. If you counted 163 colonies on Plate B,
what is the concentration of cells/ml in the original culture?
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