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You are given a culture tube labeled X as your unknown. Outline the 7 steps that will enable you to determine whether it is gram + or gram -.
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- You are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flaskWhat is the microbiology laboratory test that Identifies Pseudomonas masseli and what are the results of the tests that identify it? please include your sources in MLA. examples are hemolytic tests, lipid concentrations, genomic tests etc.What is the microbiology laboratory test that Identifies Pseudomonas putida and what are the results of the tests that identify it? please include your sources in MLA. examples are hemolytic tests, lipid concentrations, genomic tests etc.
- What is the microbiology laboratory test that Identifies Pseudomonas soli and what are the results of the tests that identify it? please include your sources in MLA. examples are hemolytic tests, lipid concentrations, genomic tests etc.Describe the process to make a 4-phase streak plate beginning with your first streak (i.e you have bacterial culture on your sterile loop and are about to start to streak your agar plate ).What is the shape of the bacteria in the pictures under microscope ( Cocci or Bacilli ) and why with explanation? These bacteria types gram positive or gram negative and why ? Please compare and discuss of these bacterias the shape and gram positive or negative according to web resources. State your reasons why you think the shape and gram positivity of both bacterias Are those images OK? OR if there is something wrong in the images what is it? What is the problem? What could it be done for getting more quality images in Gram staining? What do we pay attention in Gram staining?
- What is the microbiology laboratory test that Identifies Pseudomonas plecoglossicida and what are the results of the tests that identify it? please include your sources in MLA. examples are hemolytic tests, lipid concentrations, genomic tests etc.What is the microbiology laboratory test that Identifies Pseudomonas maumuensis and what are the results of the tests that identify it? please include your sources in MLA. examples are hemolytic tests, lipid concentrations, genomic tests etc.Which of the following plates (dilution factor) would you count/use to determine the original bacterial count? 1 ml Original inoculum Dilutions Plating 9 ml broth in each tube 1:10 1:10 1 ml 1:100 2 (1:100) 3 (1:1000) 4 (1:10,000) 5 (1:100,000) 1 ml 1:100 1:1000 ml 1 ml 1:1000 1:10,000 1 ml 1 ml 1:10,000 1:100,000 ml 1:100,000 Calculation: Number of colonies on plate x reciprocal of dilution of sample number of bacteria/ml (For example, if 32 colonies are on a plate of 10.000 dilution, then the count is 32 x 10,000=320,000/ml in sample.) Copyright © 2004 Pearson Education Inc.publishing a Benjenin Cummings
- Which of the following plates (dilution factor) would you count/use to determine the original bacterial count? 1 ml Original inoculum Dilutions Plating 1:10 9 ml broth in each tube 1:10 1 ml 1 ml 1 ml 1:100 1 ml 1:100 2 (1:100) 3 (1:1000) 4 (1:10,000) 5 (1:100,000) 1:1000 1 ml 1 ml 1:1000 1:10,000 1 ml 1 ml 1:10,000 1:100,000 1 ml 1:100,000 Calculation: Number of colonies on platex reciprocal of dilution of sample=number of bacteria/ml (For example, if 32 colonies are on a plate of 1/10,000 dilution, then the count is 32 x 10,000 = 320,000/ml in sample.) Copyright © 2004 Pearson Education, Inc., publishing as Benjamin CummingsAverage plaques for bacteriophage A,B,C are 137,36,25. PFU/ml is average plaques multiply by the volume dilution multiply by the dilution factor. Show your working for each bacteriophage.At the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No