n 1- Question 14 ation of cells and cellular components is accomplished with a centrifuge. A sample of cells is first blended to mechanically break apart some of the cells releasing their contents to form a cell te. The remaining whole cells and cell components are separated based on their sedimentation rate by centrifuging the sample at increasingly high speed. The centrifuge spins at up to several rotations per minute (rpm) forcing material to accumulate as a pellet at the bottom of the tube. The liquid portion is removed and centrifuged again to yield four (4) pellets containing the cells or cell ts listed in the diagram. Low speed wate 1 2 3 = Medium speed Pellet 1 Whole cells Nuclei Cytoskeletons 1 .. High speed ration of whole cells is required for the diagnosis and treatment of certain disease processes in humans. Four patients each exhibiting unique symptoms are shown in the table. The symptoms of ent can be explained based on damage to one, and only one, organelle or cell structure. Pellet 2 Mitochondria Lysosomes Peroxisomes Very high speed Pellet 3 Microsomes Other small vesicles Pellet 4 Ribosomes Viruses Large macromolecules Some of the symptoms In the patients Body aches (Fabry disease), due to accumulation of proteins and fat in cells. Lack of alpha-galactosidase enzyme and other digestive enzymes. Muscle fiber weakness; exercise intolerance, multiple organ failure. Incomplete fingernails due to low keratin protein; un-pigmented spots on skin, due to low melanin protein production (DKC disease). Damaged cell function and shape due to loss of desmin and other filaments network. TWO options would doctors expect to find evidence explaining the symptoms experienced by each patient? Pellets 1 and 3 because it's likely that the patients have damage in their cytoskeleton, microsomes, and other small vesicles. Pellets 1, 2 and 3 because it's likely that the patients have damage in their cytoskeleton, mitochondria, and microsomes. Pellets 1, 2 and 4 because it's likely that the patients have damage in their cytoskeleton, peroxisomes, and ribosomes. Pellets 2 and 3 because it's likely that the patients have damage in their mitochondria, lysosomes, and microsomes. Pellets 2 and 4 because it's likely that the patientshave damage in their lysosomes, mitochondria, and ribosomes.