When combining 0.5 mL of Lysozyme solution (with a concentration of 10 mg/ml) and 0.5 mL of water, what is the final concentration of the Lysozyme solution after adding 4.0 mL of biuret reagent to the solution?
When combining 0.5 mL of Lysozyme solution (with a concentration of 10 mg/ml) and 0.5 mL of water, what is the final concentration of the Lysozyme solution after adding 4.0 mL of biuret reagent to the solution?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
5

Transcribed Image Text:QUESTION 5
When combining 0.5 mL of Lysozyme solution (with a concentration of 10 mg/ml) and 0.5 mL of water, what is the final
concentration of the Lysozyme solution after adding 4.0 mL of biuret reagent to the solution?
O 1 mg/mL
O 5 mg/mL
O 10 mg/mL
O 100 mg/mL
Expert Solution

Step 1
Lysozyme is an enzyme which catalyzes the hydrolysis of peptidoglycans present in cell walls of bacteria. When used along with water and other reagents, its concentration will decrease as there will be less enzyme molecules per unit volume of the reaction mixture.
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