Mitflin and associates described a membrane electrode for the quantitative analysis of penicillin in which the enzyme penicillinase is immobilized in a polyacrylamide gel that is coated on a glass RH electrode. The following data were collected for a series of penicilin standards. [Penicillin) (M) 1.0x10? 2.0x10 1.0x10 2.0x10 1.0x10* Potential (mV) 215 199 185 148 130

Introduction to Chemical Engineering Thermodynamics
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Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
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Chapter1: Introduction
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Mitflin and associates described a membrane electrode for the quantitative analysis of
penicilin in which the enzyme penicilinase is immobilized in a polyacrylamide gel that is
coated on a glass Rl electrode, The following data were collected for a series of penicilin
standards.
Potential
(mV)
[Penicillin]
(M)
1.0x102
2.0x103
1.0x103
2.0x104
215
199
185
148
1.0x104
1.0x105
1.0x106
130
91
75
Construct a çalibration curve for the electrode, and report
(a) the range of concentrations in which a linear response is observed,
(b) the equation for the calibration curve in this range, and
(c) the concentration of penicillin in a sample that yields a potential of 138 mV,
Transcribed Image Text:Mitflin and associates described a membrane electrode for the quantitative analysis of penicilin in which the enzyme penicilinase is immobilized in a polyacrylamide gel that is coated on a glass Rl electrode, The following data were collected for a series of penicilin standards. Potential (mV) [Penicillin] (M) 1.0x102 2.0x103 1.0x103 2.0x104 215 199 185 148 1.0x104 1.0x105 1.0x106 130 91 75 Construct a çalibration curve for the electrode, and report (a) the range of concentrations in which a linear response is observed, (b) the equation for the calibration curve in this range, and (c) the concentration of penicillin in a sample that yields a potential of 138 mV,
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