In a FRET experiment designed to monitor conformational changes in T4 lysozyme, the fluorescence intensity fluctuates between 5000 and 10,000 counts per second. Assuming that 7500 counts represents a FRET efficiency of 0.6, what is the change in FRET pair separation distance during the reaction? For the tetramethylrhodamine-texas red FRET pair employed ro = 50. Å. Express your answer to two significant figures and include the appropriate units. Ar= μA Value Units ?

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