Problem 3. (6.27, From Chang's Book). The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment: Total Ca2+ (uM) 60 120 180 240 480 Ca2+ bound to Protein (uM) 31.2 51.2 63.4 70.8 83.4 Determine graphically the dissociation constant of the Ca2+ - protein complex. The protein concentration was kept at 96 µM for each run. (1 uM = 1 x 10-6 M.)
Problem 3. (6.27, From Chang's Book). The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment: Total Ca2+ (uM) 60 120 180 240 480 Ca2+ bound to Protein (uM) 31.2 51.2 63.4 70.8 83.4 Determine graphically the dissociation constant of the Ca2+ - protein complex. The protein concentration was kept at 96 µM for each run. (1 uM = 1 x 10-6 M.)
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
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Transcribed Image Text:Problem 3. (6.27, From Chang's Book). The calcium ion binds to a certain protein to form a 1:1 complex. The following data
were obtained in an experiment:
Total Ca2+ (uM)
60
120
180
240
480
Ca2+ bound to Protein (uM)
31.2 51.2 63.4
70.8
83.4
Determine graphically the dissociation constant of the Ca2+ - protein complex. The protein concentration was kept at 96 µM
for each run. (1 uM = 1 x 10-6 M.)
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