min+ = confers resistance to minocycline cep+ = confers resistance to cephalexin van+ = confers resistance to vancomycin The following table shows the results of your experiments. Transformation of the recipient cells was conducted, using the DNA of the donor cells. You next selected for recipient cells that had undergone transformation by plating the transformed cells on media containing minocycline. This enabled you to select for min+ cells. The min+ cells were then replica plated onto media containing cephalexin or vancomycin in order to determine the antibiotic resistance phenotypes with respect to these other two antibiotics Questions a) Of the three potential orders of these genes, which orders are possible and which are impossible based on your knowledge of the relative distance? b) Finally, determine the correct order of the three genes. Please include a
min+ = confers resistance to minocycline
cep+ = confers resistance to cephalexin
van+ = confers resistance to vancomycin
The following table shows the results of your experiments. Transformation of the recipient cells was conducted, using the DNA of the donor cells. You next selected for recipient cells that had undergone transformation by plating the transformed cells on media containing minocycline. This enabled you to select for min+ cells. The min+ cells were then replica plated onto media containing cephalexin or vancomycin in order to determine the antibiotic resistance
Questions
a) Of the three potential orders of these genes, which orders are possible and which are impossible based on your knowledge of the relative distance?
b) Finally, determine the correct order of the three genes. Please include a DIAGRAM to illustrate how you deduced the correct gene order, as well as an explanation of your reasoning.
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