Answered: microvilli z plane сгуpts 4 days Figure…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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At any given time, intestinal crypts of mice com-
prise about 15 stem cells and 10 Paneth cells. After cell
division, which occurs about once a day, the daughter
cells remain stem cells only if they maintain contact with a
Paneth cell. This constant competition for Paneth-cell con-
tact raises the possibility that crypts might become mono-
clonal over time; that is, the crypt cells at one point in time
might derive from only 1 of the 15 stem cells that existed
at some earlier time. To test this possibility, you use the
so-called confetti marker that upon activation expresses
any one of three fluorescent proteins in the stem cells of
the crypt. You then examine crypts at various times to
determine whether they contain cells with multiple colors
or only one color (Figure Q22–1). Do the crypts become
monoclonal over time or not? How can you tell?

microvilli
z plane
сгуpts
4 days
Figure Q22-1 Fluorescent cells
in crypts in mouse intestines at
various times after activation of
expression of fluorescent proteins
(Problem 22-6). The images
are taken in the xz plane, which
cuts through multiple crypts,
as indicated in the schematic
drawing. Roughly 50 crypts are
visible in each section. Dotted
white circles identify some
individual crypts. Scale bars are
100 um. (Adapted from
H.J. Snippert et al., Col
143:134-144, 2010. With
permission from Elsevier.)
4 weeks
30 weeks

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