Log Reduction 1000000000 100000000 10000000 1000000 100000 10000 1000 100 10 1 Time (minutes) 1. Use the above graph to determine an approximate D80 for E. coli in apple juice. B 2 min C 3 min D4 min A 1 min Need more information

Log Reduction 1000000000 100000000 10000000 1000000 100000 10000 1000 100 10 1 Time (minutes) 1. Use the above graph to determine an approximate D80 for E. coli in apple juice. B 2 min C 3 min D4 min A 1 min Need more information

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Nutritional requirements of white verses red yeast What is the differences in the ability of different strains to grow on different mediums. Propose a hypothesis that helps to explain the presence of red yeast colonies. Why it is more common for cells to be killed by UV light than to become red. Hint: think about how mutagenesis occurs.
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D Question 9 An enzyme assay of 2 mL of extract shows 7.3 U/mL and a biuret assay shows 4 mg/mL protein on the initial sample. It is salted out and the pellet is washed and resuspended in 0.4 mL buffer. It is re-assayed and shows 12.1 U/mL activity and 1.3 mg/mL protein. What is the change in fold purity for this sample? (answer to 2 decimal places)
I just don't know how to get the answer for C and D. Expecially for C, why F+died because of leu-?
use these OD numbers to plot growth curve for E. coli K12- and estimate generation time for this culture. Table 1. Absorbance (O.D.600) measured for growing culture of E. coli K12 (time course) Incubation time O.D.600 Incubation time O.D.600 O min 3 hrs 3 hrs 30 min 4 hrs 4 hrs 30 min 5 hrs 5 hrs 30 min 0.11 1.75 30 min 0.20 1.94 1 hr 1 hr 30 min 2 hrs 2 hrs 30 min 0.27 2.24 0.51 2.48 2.31 2.19 0.65 1.30
For the study of alanine production by a recombinant strain of E. coli, cultivation was carried out in a benchtop bioreactor with 4.5 L of culture medium, using glucose as a limiting substrate. During the cultivation, there was no lag phase and the cells showed exponential growth for 5 hours. The following table presents the results of the analysis of ammonia and glucose consumption, and alanine accumulation throughout the cultivation. Knowing that 500 mL of a cell suspension at a concentration of 5.0 g/L (inoculum) was added to the 4.5 L of medium in the reactor and that the YX/NH3 previously determined was 7.5, calculate:a) the maximum specific growth rateb) YX/S and YP/S yield factorsc) How long would it take to reach Cx = 30 g/L if the cells continued with the exponential growth profile until the end of the culture (without nutrient deprivation or any type of inhibition)?d) Describe how the mathematical treatment of the data should be done to determine the type of product formation…
In the experiments below, different prokaryotic cells were grown in broth cultures in the presence or absence of an antibiotic (either vancomycin or polymixin) After 1 hour, cells were rinsed, and the percent of bacteria that survived was determined. According to the data, which experiment (A or B) was done with vancomycin and which was done with polymyxin? Explain your reasoning in both cases. 100 0 A Bacillus E. coli with antibiotic without antibiotic B Methanoge n 100 Bacillus E. coli Methanogen
Prepare a dilution of phage from 1.5 x 1010 PFU/mL to 1.00 x 102 PFU/mL. Draw a dilution scheme to achieve this serial dilution considering the following: The volumes of each dilution should be 1.0mL. It is not advisable to perform any dilutions > 1/100. The first dilution in any titre determination of stock phage should use 10µl or less of stock.
Content × Content / Ana ☑ OntarioLearn × Content × Furosemide is ☑ ChatGPT × G Intradermal in × + ☑ ← >> https://ontariolearn.blackboard.com/ultra/courses/_30758_1/cl/outline?legacyUrl=~2Fwebapps~2Fblackboard~2Fcontent~2Flaunch...✰ S Relaunch to update : M Gmail ►▸ YouTube Maps Sign in | Permanent... Search Results login... GMy Grammarly - Gr... York Region Childre... Applications Home | bartleby (50,482 unread) - a... Remaining Time: 13 minutes, 18 seconds. * Question Completion Status: 1 points ✓ Saved QUESTION 6 Furosemide is a loop diuretic used in the treatment of edema and hypertension. It is available with a strength of 20 mg/tab. A prescriber has ordered a daily dose of 40 mg. Which of the following configurations will yield the correct answer to the dosage calculation? 40mg 1 20 mg ✗ 1 tab ○ 40mg 1 tab 1 20 mg E 1 1 tab ✗ 40mg 20 mg อ 1 40mg 20 mg ✗ 1 tab Priva Tern Acce 1 Click Save and Submit to save and submit. Click Save All Answers to save all answers. Q Search myhp W…
Draw a dilution scheme for a dilution of phage from 1.5 x 1010 PFU/mL to 1.00 x 102 PFU/mL. Achieve this serial dilution considering the following: The volumes of each dilution should be 1.0mL. It is not advisable to perform any dilutions > 1/100. The first dilution in any titre determination of stock phage should use 10µl or less of stock.
A 3.0 L batch bioreactor is used to grow E. coli bacteria, which have an initial concentration of 12.5 g cells/L. The growth medium contains two carbon sources, which causes diauxic growth, with each carbon source limiting cell growth. Sufficient supplies of oxygen, ammonium nitrate (NH4NO3) as a nitrogen source and all other macro and micronutrients are available. The bacteria prefer to grow on glucose (C6H12O6, supplied at 80 g/L), so it is consumed first, followed by glycerol (C3H803, supplied at 68 g/L). Since the E. coli were already growing on glucose, the lag time associated with glucose consumption is only 10 minutes. The lag time associated with glycerol consumption is 35 minutes. Further information: The chemical formula for E. coli in this bioreactor is CH1.666N0.2000.57. The specific growth rate on glucose is 0.41 h-1, and on glycerol is 0.32 h-1?The yield of cells on glucose is 0.48 g cells/g glucose, and yield on glycerol is 0.39 g cells/g glycerol. You may assume that…
The data below were obtained for the growth of a pure culture of Escherichia coli in nutrient broth at a temperature of 37°C. Determine (1) the specific growth rate and (2) generation time of E. coli and the duration of the (3) lag and (4) log (or exponential) phases. (ln 2 = 0.693) Time (h) 0 1 2 3 4 8 16 32 Bacterial No./mL 104.1 103.9 104.4 105.5 106.5 107.7 108.0 107.6