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I just don't know how to get the answer for C and D. Expecially for C, why F+died because of leu-?

I just don't know how to get the answer for C and D. Expecially for C, why F+died because of leu-?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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I just don't know how to get the answer for C and D. Expecially for C, why F+died because of leu-? 

Sample Problem 3. Conjugations are set up between E. coli of the genotypes indicated below. Bacteria are incubated for 10 minutes and then plated on minimal media with a concentration of azide (azi) sufficient to kill azi-sensitive E. coli) Predict how many Hfr, F+ and F- colonies (many, few, none will appear on the azi plates and how they would come about. Note: the leu locus is very near the origin of transfer, whereas the azi locus is not. to kill donor strain lent A. Hfr azieu+x_F-azi' leu- B. Hfr azileu-x F-azileut -C. Hfr azis leu+ x F+ azi¹ leu- D. F+ azi leu+ x F- azi¹ leu- H fr F* A, none B. none С. попе D. none. none none none none chrom Dintegration creates Mfr (3) FF many due to Fplasmid conjugation Es donates leu many because many. bacteria will not conjugate none few because 2 steps mus happen.
Transcribed Image Text:Sample Problem 3. Conjugations are set up between E. coli of the genotypes indicated below. Bacteria are incubated for 10 minutes and then plated on minimal media with a concentration of azide (azi) sufficient to kill azi-sensitive E. coli) Predict how many Hfr, F+ and F- colonies (many, few, none will appear on the azi plates and how they would come about. Note: the leu locus is very near the origin of transfer, whereas the azi locus is not. to kill donor strain lent A. Hfr azieu+x_F-azi' leu- B. Hfr azileu-x F-azileut -C. Hfr azis leu+ x F+ azi¹ leu- D. F+ azi leu+ x F- azi¹ leu- H fr F* A, none B. none С. попе D. none. none none none none chrom Dintegration creates Mfr (3) FF many due to Fplasmid conjugation Es donates leu many because many. bacteria will not conjugate none few because 2 steps mus happen.
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