L-ornithine 2 carbamoylphosphate 3 L-citrulline 4 argininosuccinate 5 fumarato 6 L-arginine 7 urea L-Asp L-aspartate CPS-1 carbamoyl phosphate synthetase OTC Omnithine transcarbamoylase ASS argininosuccinate synthetase ASL argininosuccinate lyase ARG1 arginase 1 OH NH₂ H₂N NH CHI ASL CH₂ Coo CH₂ CH₂ coo Which of the reactions is requires energy input? (Answer by choosing the enzyme used)? 2ATPHCONH₂ 2 ADP. PCPS-1 H₂N ARG1 H₂O отс fessels - -8-5-9-8- CH₂ ASS H.M.000 ATP PP, L-Asp AMP

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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**Educational Content: Urea Cycle Reactions**

The diagram illustrates the urea cycle, a crucial metabolic pathway for the detoxification of ammonia in the liver. The cycle involves a series of biochemical reactions that convert toxic ammonia to urea, which is excreted from the body.

### Key Components and Steps in the Urea Cycle:

1. **L-ornithine to carbamoyl phosphate**
   - Enzyme: Carbamoyl phosphate synthetase I (CPS-I)
   - Reactants: 2 ATP, HCO₃⁻, NH₄⁺
   - Products: 2 ADP, P_i, Carbamoyl phosphate

2. **Ornithine transcarbamylase (OTC) reaction**
   - Enzyme: Ornithine transcarbamylase (OTC)
   - Substrate: Carbamoyl phosphate
   - Product: Citrulline
   - Transport: Citrulline is transported out of the mitochondria

3. **Argininosuccinate synthetase (ASS) reaction**
   - Enzyme: Argininosuccinate synthetase (ASS)
   - Reactants: Citrulline, Aspartate, ATP
   - Products: Argininosuccinate, AMP, PP_i

4. **Argininosuccinate lyase (ASL) reaction**
   - Enzyme: Argininosuccinate lyase (ASL)
   - Product: Arginine, Fumarate

5. **Arginase 1 (ARG1) reaction**
   - Enzyme: Arginase 1 (ARG1)
   - Product: Urea, Ornithine
   - Ornithine is then recycled back into the mitochondria for another cycle.

### Graphical Explanation:
- The left part of the diagram represents the mitochondrial membrane.
- The reactions within the membrane illustrate the synthesis of citrulline inside the mitochondria.
- The extra-mitochondrial reactions show the conversion of citrulline through subsequent steps eventually leading to urea.
- Each enzyme catalyzing a specific reaction is denoted next to the reaction step.

### Question:
Identify which of the reactions require energy input (Answer by choosing the enzyme used):

- OTC
- ASS
- ASL
- ARG1
- NONE of these require energy to do their reaction

The answer involves
Transcribed Image Text:**Educational Content: Urea Cycle Reactions** The diagram illustrates the urea cycle, a crucial metabolic pathway for the detoxification of ammonia in the liver. The cycle involves a series of biochemical reactions that convert toxic ammonia to urea, which is excreted from the body. ### Key Components and Steps in the Urea Cycle: 1. **L-ornithine to carbamoyl phosphate** - Enzyme: Carbamoyl phosphate synthetase I (CPS-I) - Reactants: 2 ATP, HCO₃⁻, NH₄⁺ - Products: 2 ADP, P_i, Carbamoyl phosphate 2. **Ornithine transcarbamylase (OTC) reaction** - Enzyme: Ornithine transcarbamylase (OTC) - Substrate: Carbamoyl phosphate - Product: Citrulline - Transport: Citrulline is transported out of the mitochondria 3. **Argininosuccinate synthetase (ASS) reaction** - Enzyme: Argininosuccinate synthetase (ASS) - Reactants: Citrulline, Aspartate, ATP - Products: Argininosuccinate, AMP, PP_i 4. **Argininosuccinate lyase (ASL) reaction** - Enzyme: Argininosuccinate lyase (ASL) - Product: Arginine, Fumarate 5. **Arginase 1 (ARG1) reaction** - Enzyme: Arginase 1 (ARG1) - Product: Urea, Ornithine - Ornithine is then recycled back into the mitochondria for another cycle. ### Graphical Explanation: - The left part of the diagram represents the mitochondrial membrane. - The reactions within the membrane illustrate the synthesis of citrulline inside the mitochondria. - The extra-mitochondrial reactions show the conversion of citrulline through subsequent steps eventually leading to urea. - Each enzyme catalyzing a specific reaction is denoted next to the reaction step. ### Question: Identify which of the reactions require energy input (Answer by choosing the enzyme used): - OTC - ASS - ASL - ARG1 - NONE of these require energy to do their reaction The answer involves
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