In order to obtain better separation of the mixture, the above analysis was repeated using an HPLC technique. The retention times of compounds A and B were found to be 15.25 and 16.80 min, respectively, on a 40-cm packed column. An unretained species passed through the column in 1.25 min. The peak base width for A and B are 1.15 and 1.27 min, respectively. Calculate: i) The column resolution. ii) The average number of plates in the column. iii) The plate height. iv) The length of column required to achieve a resolution of 1.5.
In order to obtain better separation of the mixture, the above analysis was repeated using an HPLC technique. The retention times of compounds A and B were found to be 15.25 and 16.80 min, respectively, on a 40-cm packed column. An unretained species passed through the column in 1.25 min. The peak base width for A and B are 1.15 and 1.27 min, respectively. Calculate: i) The column resolution. ii) The average number of plates in the column. iii) The plate height. iv) The length of column required to achieve a resolution of 1.5.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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In order to obtain better separation of the mixture, the above analysis was repeated using an HPLC technique. The retention times of compounds A and B were found to be 15.25 and 16.80 min, respectively, on a 40-cm packed column. An unretained species passed through the column in 1.25 min. The peak base width for A and B are 1.15 and 1.27 min, respectively. Calculate:
i) The column resolution.
ii) The average number of plates in the column.
iii) The plate height.
iv) The length of column required to achieve a resolution of 1.5.
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