Nonpolar aromatic compounds were separated by HPLC on an octadecyl(C18) bonded phase. The mobile phase was 65 vol% methanol in water. How would the retention times of these aromatic compounds be affected if 90 vol% methanol was used instead? The retention times will be longer in 90 vol% methanol. The retention times will double. The retention times will not change. The retention times will be shorter in 90 vol% methanol.
Nonpolar aromatic compounds were separated by HPLC on an octadecyl(C18) bonded phase. The mobile phase was 65 vol% methanol in water. How would the retention times of these aromatic compounds be affected if 90 vol% methanol was used instead? The retention times will be longer in 90 vol% methanol. The retention times will double. The retention times will not change. The retention times will be shorter in 90 vol% methanol.
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter27: Gas Chromatography
Section: Chapter Questions
Problem 27.19QAP
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![Nonpolar aromatic compounds were separated by HPLC on an octadecyl(C18)
bonded phase. The mobile phase was 65 vol% methanol in water. How would the
retention times of these aromatic compounds be affected if 90 vol% methanol was
used instead?
The retention times will be longer in 90 vol% methanol.
The retention times will double.
The retention times will not change.
The retention times will be shorter in 90 vol% methanol.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F4787dfbd-45a2-4b25-8dde-45ae660a9830%2F35da80b4-dd38-4b91-aa42-2d6fcf1d49f8%2Frwa7v68.bmp&w=3840&q=75)
Transcribed Image Text:Nonpolar aromatic compounds were separated by HPLC on an octadecyl(C18)
bonded phase. The mobile phase was 65 vol% methanol in water. How would the
retention times of these aromatic compounds be affected if 90 vol% methanol was
used instead?
The retention times will be longer in 90 vol% methanol.
The retention times will double.
The retention times will not change.
The retention times will be shorter in 90 vol% methanol.
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