An analysis of acid mixture was conducted using reversed-phase liquid chromatography with 20 mL/min flowrates and 10 cm column length. The retention time of Y acid was 4.4 min with a width of 1.6 min. While for acid Z, the retention time recorded was 6.8 min with a 1.2 min-width baseline. A non-retained compound appeared at 0.8 min after injection. Calculate the capacity factor (k') for acid Z i) Find the resolution of acid Y and Z ii) Determine the average theoretical plates in the column

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An analysis of acid mixture was conducted using reversed-phase liquid chromatography
with 20 mL/min flowrates and 10 cm column length. The retention time of Y acid was 4.4
min with a width of 1.6 min. While for acid Z, the retention time recorded was 6.8 min with
a 1.2 min-width baseline. A non-retained compound appeared at 0.8 min after injection.
i)
Calculate the capacity factor (k') for acid Z
ii) Find the resolution of acid Y and Z
iii) Determine the average theoretical plates in the column
Transcribed Image Text:An analysis of acid mixture was conducted using reversed-phase liquid chromatography with 20 mL/min flowrates and 10 cm column length. The retention time of Y acid was 4.4 min with a width of 1.6 min. While for acid Z, the retention time recorded was 6.8 min with a 1.2 min-width baseline. A non-retained compound appeared at 0.8 min after injection. i) Calculate the capacity factor (k') for acid Z ii) Find the resolution of acid Y and Z iii) Determine the average theoretical plates in the column
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