Answers po sana for letters D, E & F 1. The following data are for a liquid chromatographic column:length of packing: 24.7 cmVM : 1.37 mLFlow rate: 0.313 mL /minVs: 0.164 mlA chromatogram of a mixture of species A, B, C, and D provided the following data:Retention time, min3.15.4Width of Peak Base (W), minNonretained13.314.121.60.411.071.161.72Calculate: (a) the number of plates from each peak.(b) the mean and standard deviation for N.(c) the plate height for the column.(d) the capacity factor for A, B, C, and D.(e) the resolution for species B and C.(f) the length of column necessary to separate species C and D with a resolution of 1.5.ABCD

(d) Capacity factor (k) is given as: k=tr-tmtmwhere,tm is the retention time of nonretained solute

Answered: (d) the capacity factor for A, B, C,…

(d) the capacity factor for A, B, C, and D. (e) the resolution for species B and C. (f) the length of column necessary to separate species C and D with a resolution of 1.5.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

Answers po sana for letters D, E & F

1. The following data are for a liquid chromatographic column:
length of packing: 24.7 cm
VM : 1.37 mL
Flow rate: 0.313 mL /min
Vs: 0.164 ml
A chromatogram of a mixture of species A, B, C, and D provided the following data:
Retention time, min
3.1
5.4
Width of Peak Base (W), min
Nonretained
13.3
14.1
21.6
0.41
1.07
1.16
1.72
Calculate: (a) the number of plates from each peak.
(b) the mean and standard deviation for N.
(c) the plate height for the column.
(d) the capacity factor for A, B, C, and D.
(e) the resolution for species B and C.
(f) the length of column necessary to separate species C and D with a resolution of 1.5.
ABCD

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