Polar compounds were separated by HPLC using a bonded phase containing polar diol substituent [-CH(OH)CH,OH]. How would the retention times be affected if the eluent were changed from 70 vol.% to 30 vol.% of acetonitrile in water? Retention times will be unchanged. Retention times will be shorter Retention times will be longer D Changes in retention times are not sure.
Polar compounds were separated by HPLC using a bonded phase containing polar diol substituent [-CH(OH)CH,OH]. How would the retention times be affected if the eluent were changed from 70 vol.% to 30 vol.% of acetonitrile in water? Retention times will be unchanged. Retention times will be shorter Retention times will be longer D Changes in retention times are not sure.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![Polar compounds were separated by HPLC using a bonded phase containing polar diol
substituent [-CH(OH)CH2OH]. How would the retention times be affected if the eluent
were changed from 70 vol.% to 30 vol.% of acetonitrile in water?
O Retention times will be unchanged.
Retention times will be shorter
KO Retention times will be longer
O Changes in retention times are not sure.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9d294bda-2c8b-4008-be6d-440213cedbe3%2F1e75ada8-d0fa-494c-8d70-cf420d45887f%2Ftgf6bzq_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Polar compounds were separated by HPLC using a bonded phase containing polar diol
substituent [-CH(OH)CH2OH]. How would the retention times be affected if the eluent
were changed from 70 vol.% to 30 vol.% of acetonitrile in water?
O Retention times will be unchanged.
Retention times will be shorter
KO Retention times will be longer
O Changes in retention times are not sure.
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