An analyst wants to separate two compounds A and B using HPLC. Compound A has a retention time of 3 minutes and compound B has a retention time of 5 minutes. If an HPLC column with a length of 150 mm and an internal diameter of 4.6 mm is used, and a mobile phase composed of a mixture of water and methanol in a 70:30 ratio is used, calculate what the velocity would be? adequate flow rate to obtain a good separation of the two compounds?

What would happen to the retention time of a given compound if the carrier gas flow rate of the gas chromatograph is increased?      The retention time would increase.      The retention time would decrease.      The retention time would not change.

a. Say you are trying to separate two compounds by column chromatography. You run a column and collect the eluent in five fractions. You analyze each fraction by TLC on the same plate (shown below). Based on the TLC plate, how effective was your separation? Consider the purity of each fraction. - - - - - - - - ----| 1 2 3 4 5

What are the retention times and areas of all the attached peaks?

Sample contains (A, B, C, D) analyzed by thin layer chromatography using solvent system X the result showed:Rf Values: (A: 0.6, B: 0.9, C: 0.5, D: remained in the base line).1) If the sample was separated by using column chromatography. Which of these substances eluted first? And what is the detecting method?2) If the sample was separated by using HPLC (1.Normal phase 2. Reversed phase) which RT of these substances comes first of both phases? And what is the ?meaning of RT

In the table below, record the color that you observe for each spot or component of each spot after the run is completed. For the retention factor, R₁, write in the numerator (top) the number of mm that the spot has moved and in the denominator (bottom) the number of mm that the solvent has moved from the point where the samples were 'spotted. Calculate the Rf vale for each spot or component of each spot. Im having a difficult time finding the Retention Factor for M&Ms by using Salt water and seeing how much each color moves Instructions Coffee filters usually are round, but it's easier to compare your results if the paper is rectangular. So, your first task is to cut the coffee filter into a rectangle. Measure and cut a 2-3/4" by 7" strip from the middle of a coffee filter. This is easily done by folding the filter in half (to make a half-circle) and then folding the half-circle into thirds. Cut along the 13-fold lines and trim the ends to make a rectangle. 2. Using a pencil (ink from…

How GC retention time can be affected by the mobile phase flow rate and the temperature of the column

A mixture of species of A, B, and C is injected into a liquid chromatographic column with length of 20.0 cm. The obtained separation results are listed as follows: Please calculate the resolution for species B and C from the data. Please keep your result to two decimal places. The answer would be 1.22, I would need help how to get that answer

Two compounds A and B from GC on two open tubular columns contains a XY-1 (100% methyl) and a XY-2 (50% methyl, 50% phenyl), with the same diameter, thickness and length under the same experimental conditions. The retention times and peak widths are show in the Table 1.Hint: phenyl groups interact more strongly with polar groups than methyl groups. Which compound is more polar? Explain your answer