An analyst wants to separate two compounds A and B using HPLC. Compound A has a retention time of 3 minutes and compound B has a retention time of 5 minutes. If an HPLC column with a length of 150 mm and an internal diameter of 4.6 mm is used, and a mobile phase composed of a mixture of water and methanol in a 70:30 ratio is used, calculate what the velocity would be? adequate flow rate to obtain a good separation of the two compounds?

Component 1 on a GC column elutes before component 2. Which of the following statements is true? Select one: a. The adjusted retention time is larger than the unadjusted retention time. b. Component l has more affinity for the stationary phase than component 2. c. Component 1 has more affinity for the mobile phase than component 2. d. The relative retention a (alpha), is always <1. е. The adjusted retention time of component 1 is larger than that for component 2.

What would happen to the retention time of a compound if the following changes were made? 1.) Decrease the flow rate of the carrier gas 2.) Increase the temperature of the column 3.) Increase the length of the column

In the table below, record the color that you observe for each spot or component of each spot after the run is completed. For the retention factor, R₁, write in the numerator (top) the number of mm that the spot has moved and in the denominator (bottom) the number of mm that the solvent has moved from the point where the samples were 'spotted. Calculate the Rf vale for each spot or component of each spot. Im having a difficult time finding the Retention Factor for M&Ms by using Salt water and seeing how much each color moves Instructions Coffee filters usually are round, but it's easier to compare your results if the paper is rectangular. So, your first task is to cut the coffee filter into a rectangle. Measure and cut a 2-3/4" by 7" strip from the middle of a coffee filter. This is easily done by folding the filter in half (to make a half-circle) and then folding the half-circle into thirds. Cut along the 13-fold lines and trim the ends to make a rectangle. 2. Using a pencil (ink from…