An analyst wants to separate two compounds A and B using HPLC. Compound A has a retention time of 3minutes and compound B has a retention time of 5 minutes. If an HPLC column with a length of 150 mm andan internal diameter of 4.6 mm is used, and a mobile phase composed of a mixture of water and methanol ina 70:30 ratio is used, calculate what the velocity would be? adequate flow rate to obtain a good separationof the two compounds?

To calculate the velocity and flow rate required to obtain a good separation of compounds A and B…

Answered: An analyst wants to separate two…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Component 1 on a GC column elutes before component 2. Which of the following statements is true? Select one: a. The adjusted retention time is larger than the unadjusted retention time. b. Component l has more affinity for the stationary phase than component 2. c. Component 1 has more affinity for the mobile phase than component 2. d. The relative retention a (alpha), is always <1. е. The adjusted retention time of component 1 is larger than that for component 2.
What would happen to the retention time of a compound if the following changes were made? 1.) Decrease the flow rate of the carrier gas 2.) Increase the temperature of the column 3.) Increase the length of the column
In the table below, record the color that you observe for each spot or component of each spot after the run is completed. For the retention factor, R₁, write in the numerator (top) the number of mm that the spot has moved and in the denominator (bottom) the number of mm that the solvent has moved from the point where the samples were 'spotted. Calculate the Rf vale for each spot or component of each spot. Im having a difficult time finding the Retention Factor for M&Ms by using Salt water and seeing how much each color moves Instructions Coffee filters usually are round, but it's easier to compare your results if the paper is rectangular. So, your first task is to cut the coffee filter into a rectangle. Measure and cut a 2-3/4" by 7" strip from the middle of a coffee filter. This is easily done by folding the filter in half (to make a half-circle) and then folding the half-circle into thirds. Cut along the 13-fold lines and trim the ends to make a rectangle. 2. Using a pencil (ink from…
a. Say you are trying to separate two compounds by column chromatography. You run a column and collect the eluent in five fractions. You analyze each fraction by TLC on the same plate (shown below). Based on the TLC plate, how effective was your separation? Consider the purity of each fraction. - - - - - - - - ----| 1 2 3 4 5
How GC retention time can be affected by the mobile phase flow rate and the temperature of the column
A mixture of species of A, B, and C is injected into a liquid chromatographic column with length of 20.0 cm. The obtained separation results are listed as follows: Please calculate the resolution for species B and C from the data. Please keep your result to two decimal places. The answer would be 1.22, I would need help how to get that answer
The compounds biphenyl and naphthalene are presented in a mixture. Can hexane be used as a solvent to separate the compounds by column chromatography with silica gel as the stationary phase? Why or why not.
Consider the following chromatographic data for the separation of methylparaben from ethylparaben in a sunscreen sample. Compound Methylparaben Retention time, tr (min). Width at base of peak, Wb (min) 3.2 4.2 0.55 0.60 Ethylparaben A compound not retained by the column in this analysis had a tm of 0.75 minutes. The column used was a C18 column with a mobile phase mixture of 75% methanol and 25% water. Calculate the number of theoretical plates for ethylparaben.

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