A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 206.1 s with a baseline peak width, we, of 14.4 s. The retention time for aspartame, ta, was 259.3 s with a baseline peak width, wa, of 18.8 s. The retention time for the unretained solvent methanol was 46.8 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 28.0 cm long column. H = μm Calculate the resolution, R, for this separation using the widths of the peaks. R = Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5.

A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 206.1 s with a baseline peak width, we, of 14.4 s. The retention time for aspartame, ta, was 259.3 s with a baseline peak width, wa, of 18.8 s. The retention time for the unretained solvent methanol was 46.8 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 28.0 cm long column. H = μm Calculate the resolution, R, for this separation using the widths of the peaks. R = Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5.

Macroscale and Microscale Organic Experiments

7th Edition

ISBN:9781305577190

Author:Kenneth L. Williamson, Katherine M. Masters

Publisher:Kenneth L. Williamson, Katherine M. Masters

Chapter9: Column Chromatography: Fluorenone, Cholesteryl Acetate, Acetylferrocene, And Plant Pigments

Section: Chapter Questions

Problem 5Q

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A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 200.8 s with a baseline peak width, we, of 16.1 s. The retention time for aspartame, ta, was 258.7 s with a baseline peak width, wa, of 20.6 s. The retention time for the unretained solvent methanol was 44.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = Calculate the resolution, R, for this separation using the widths of the peaks. R = 88.2 R₂5N = 3.16 Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5. 3.533 Incorrect μm
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