Given that the following chromatogram for the separation of two solutes (A and B) on a 25 cm long HPLC column. The retention time of the un-retained species is 0.9 minute, compound A is 6.0 min and compound B is 7.8 min. The width of the eluting peak at the baseline for compound A is 1.5 min and for compound B is 1.4 min. Based on this chromatogram, answer the following questions: A (6 min) B (7.8 min) 1.01 (i) (ii) Absorbance 0.54 0 tm (0.9 min) His 10 15 20 time (min) Calculate the resolution and selectivity factor achieved for solutes A and B. Calculate the linear velocity of the mobile phase in the column

Given that the following chromatogram for the separation of two solutes (A and B) on a 25 cm long HPLC column. The retention time of the un-retained species is 0.9 minute, compound A is 6.0 min and compound B is 7.8 min. The width of the eluting peak at the baseline for compound A is 1.5 min and for compound B is 1.4 min. Based on this chromatogram, answer the following questions: A (6 min) B (7.8 min) 1.01 (i) (ii) Absorbance 0.54 0 tm (0.9 min) His 10 15 20 time (min) Calculate the resolution and selectivity factor achieved for solutes A and B. Calculate the linear velocity of the mobile phase in the column

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

Given that the following chromatogram for the separation of two solutes (A and B) on a 25
cm long HPLC column. The retention time of the un-retained species is 0.9 minute,
compound A is 6.0 min and compound B is 7.8 min. The width of the eluting peak at the
baseline for compound A is 1.5 min and for compound B is 1.4 min. Based on this
chromatogram, answer the following questions:
A (6 min) B (7.8 min)
1.01
(i)
(ii)
Absorbance
0.5-
0
tm (0.9 min)
10
15
20
time (min)
Calculate the resolution and selectivity factor achieved for solutes A and B.
Calculate the linear velocity of the mobile phase in the column

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