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In a 4th year honours project, Amy described short nucleotide polymorphism (SNP) on chromosome #1 of drosophila. Among this polymorphism for the DM3 locus, Amy described 2 alleles (DM1¹ and DM1): DM1C Amy then wanted to test if the DM1 SNP was linked to the gene for purple eyes. To do so, she first generated heterozygous flies by crossing true breeding red eyed flies with the DM1¹ marker with true- breeding purple eyed drosophila with the DM1 marker: Genotype of gamete of the F1 fly pr+ DM1T She then did a testcross to perform a linkage analysis between the DM3 marker and the gene for purple eyes: pr DMIC 5'..TCTTGATC..3' DM1T 3'..AGAACTAG..5' pr+ DMIC or 5.TCTCGATC.3 3'..AGAGCTAG..5' pr DM1T pr+ DMIC pr DMIT A) Complete the following table by indicating the types of gametes the heterozygous fly can produce (column 1); whether or not the gamete is of the parental type (P) or a recombinant (R) (column 2); and the genotype of the F2 progeny (column 3). pr+ DMIC pr DMIT Parental (P) or recombinant type (R)? X heterozygote! Phenotype of testcross offspring Red, DM1T & DM1C Purple, DM1C Red, DM1C Purple, DM1T & DM1C pr DMIC pr DMIC Genotype of testcross offspring # of progeny 322 318 78 82 B) Determine if the gene and marker are linked. If they are linked, provide a drawing of the chromosome map with the map units separating them.

In a 4th year honours project, Amy described short nucleotide polymorphism (SNP) on chromosome #1 of drosophila. Among this polymorphism for the DM3 locus, Amy described 2 alleles (DM1¹ and DM1): DM1C Amy then wanted to test if the DM1 SNP was linked to the gene for purple eyes. To do so, she first generated heterozygous flies by crossing true breeding red eyed flies with the DM1¹ marker with true- breeding purple eyed drosophila with the DM1 marker: Genotype of gamete of the F1 fly pr+ DM1T She then did a testcross to perform a linkage analysis between the DM3 marker and the gene for purple eyes: pr DMIC 5'..TCTTGATC..3' DM1T 3'..AGAACTAG..5' pr+ DMIC or 5.TCTCGATC.3 3'..AGAGCTAG..5' pr DM1T pr+ DMIC pr DMIT A) Complete the following table by indicating the types of gametes the heterozygous fly can produce (column 1); whether or not the gamete is of the parental type (P) or a recombinant (R) (column 2); and the genotype of the F2 progeny (column 3). pr+ DMIC pr DMIT Parental (P) or recombinant type (R)? X heterozygote! Phenotype of testcross offspring Red, DM1T & DM1C Purple, DM1C Red, DM1C Purple, DM1T & DM1C pr DMIC pr DMIC Genotype of testcross offspring # of progeny 322 318 78 82 B) Determine if the gene and marker are linked. If they are linked, provide a drawing of the chromosome map with the map units separating them.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In a 4th year honours project, Amy described short nucleotide polymorphism (SNP) on chromosome #1 of drosophila. Among this polymorphism for the DM3 locus, Amy described 2 alleles (DM1" and DM1°): 5'.TCTTGATC. 3' DM1T 3'.AGAACTAG. 5' or 5'.TCTCGATC.3' 3'.AGAGCTAG. 5' DM1C Amy then wanted to test if the DM1 SNP was linked to the gene for purple eyes. To do so, she first generated heterozygous flies by crossing true breeding red eyed flies with the DM1" marker with true- breeding purple eyed drosophila with the DM1C marker: pr+ PM1 heterozygote! pr DMIT She then did a testcross to perform a linkage analysis between the DM3 marker and the gene for purple eyes: pr+ DM1C pr PMIT pr DMIC pr DMIC X A) Complete the following table by indicating the types of gametes the heterozygous fly can produce (column 1); whether or not the gamete is of the parental type (P) or a recombinant (R) (column 2); and the genotype of the F2 progeny (column 3). Parental (P) Genotype of gamete of the F1 fly Phenotype of testcross offspring Genotype of testcross offspring # of or recombinant progeny type (R) ? pr+ DM1T Red, DM1" & DM1° 322 pr DM1C Purple, DM1° 318 pr+ DM1C Red, DM1° 78 Purple, DM1" & DM1° pr DM1T 82 B) Determine if the gene and marker are linked. If they are linked, provide a drawing of the chromosome map with the map units separating them.
Transcribed Image Text:In a 4th year honours project, Amy described short nucleotide polymorphism (SNP) on chromosome #1 of drosophila. Among this polymorphism for the DM3 locus, Amy described 2 alleles (DM1" and DM1°): 5'.TCTTGATC. 3' DM1T 3'.AGAACTAG. 5' or 5'.TCTCGATC.3' 3'.AGAGCTAG. 5' DM1C Amy then wanted to test if the DM1 SNP was linked to the gene for purple eyes. To do so, she first generated heterozygous flies by crossing true breeding red eyed flies with the DM1" marker with true- breeding purple eyed drosophila with the DM1C marker: pr+ PM1 heterozygote! pr DMIT She then did a testcross to perform a linkage analysis between the DM3 marker and the gene for purple eyes: pr+ DM1C pr PMIT pr DMIC pr DMIC X A) Complete the following table by indicating the types of gametes the heterozygous fly can produce (column 1); whether or not the gamete is of the parental type (P) or a recombinant (R) (column 2); and the genotype of the F2 progeny (column 3). Parental (P) Genotype of gamete of the F1 fly Phenotype of testcross offspring Genotype of testcross offspring # of or recombinant progeny type (R) ? pr+ DM1T Red, DM1" & DM1° 322 pr DM1C Purple, DM1° 318 pr+ DM1C Red, DM1° 78 Purple, DM1" & DM1° pr DM1T 82 B) Determine if the gene and marker are linked. If they are linked, provide a drawing of the chromosome map with the map units separating them.
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