In 2005, Frederick Blattner and his colleagues foundthat E. coli cells have a global transcriptional programthat helps them forage for better sources of carbon. Many genes, including genes needed for bacterial motility, are turned on in response to poorer carbonsources so that the bacteria can search for better nutrition. You now want to search for genes that regulatethis response. How could you use lacZ fusions to tryto identify such regulatory genes?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
In 2005, Frederick Blattner and his colleagues found
that E. coli cells have a global transcriptional program
that helps them forage for better sources of carbon. Many genes, including genes needed for bacterial motility, are turned on in response to poorer carbon
sources so that the bacteria can search for better nutrition. You now want to search for genes that regulate
this response. How could you use lacZ fusions to try
to identify such regulatory genes?
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