Immunostaining was used to investigate the expression of the Fibrillin 1 gene in muscle fibres. Twoantibodies were used, Antibody A binds to a muscle membrane protein that is unrelated to Fibrillin.Antibody B binds to the N terminus of Fibrillin 1, whilst Antibody C binds to the C terminus of Fibrillin 1.What is the best explanation for the staining pattern see in the following figureAntibody BAntibody AAntibody C A point mutation in the fibrillin gene introduced a stop codon in the proteinA gene translocation has removed the N terminus of the proteinA gene translocation has removed the chromosome encoding the fibrillin geneA tuncation mutation has removed the central region of the proteinThe C and N termini of the Fibrillin protein are unchanged by the mutationA point mutation in the fibrillin gene has changed an amino acid in the proteinA point mutation in the fibrillin gene has introduced a start codon in the proteinA truncation mutation has removed the N terminus of the proteinA point mutation in the fibrillin gene has resulted in the removal of a stop codonBoth the N and C termini regions of the protein have been deleted by a mutationA point mutation in the fibillingene has led to it not being expressed

Immunostaining is a process of staining proteins by antibodies that are covalently linked to an…

Answered: Immunostaining was used to investigate…

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter16: Adult And Pediatric Dosages Based On Body Surface Area

Section: Chapter Questions

Problem 7.2P

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The Adaptive Immune Response Is a Specific Defense Against Infection In cystic fibrosis gene therapy, scientists propose the use of viral vectors to deliver normal genes to cells in the lungs. What immunological risks are involved in this procedure?
b. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein E. To determine where the block in protein-E production occurred in each individual, the various intermediates were given to each individuals cel Is in culture. After a few weeks of growth with the intermediate, the cells were assayed for the production of protein E. The results for each individuals cells are given in the following table. A plus sign means that protein E was produced after the cells were given the intermediate listed at the top of the column. A minus sign means that the cells still could not produce protein E even after being exposed to the intermediate at the top of the column. Denote the point in the pathway in which each individual is blocked.
a. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein E. To determine where the block in protein-E production occurred in each individual, the various intermediates were given to each individuals cel Is in culture. After a few weeks of growth with the intermediate, the cells were assayed for the production of protein E. The results for each individuals cells are given in the following table. A plus sign means that protein E was produced after the cells were given the intermediate listed at the top of the column. A minus sign means that the cells still could not produce protein E even after being exposed to the intermediate at the top of the column. Draw the pathway leading to the production of protein E.
If an extra nucleotide is inserted in the first exon of the beta globin gene, what effect will it have on the amino acid sequence of the globin polypeptides? Will the globin most likely be fully functional, partly functional, or nonfunctional? Why?
The following is a portion of a protein: met-trp-tyr-arg-gly-pro-thr-Various mutant forms of this protein have been recovered. Using the normal and mutant sequences, determine the DNA and mRNA sequences that code for this portion of the protein, and explain each of the mutations. a. met-trp- b. met-cys-ile-val-val-leu-gln- c. met-trp-tyr-arg-ser-pro-thr- d. met-trp-tyr-arg-gly-ala-val-ile-ser-pro-thr-
Figure 9.17 Which of the following statements about quorum sensing is false? Autoinducer must bind to receptor to turn on transcription of genes responsible for the production of more autoinducer. The receptor stays in the bacterial cell but the autoinducer diffuses out. Autoinducer can only act on a different cell: it cannot act on the cell in which it is made. Autoinducer turns on genes that enable the bacteria to form a biofilm.
How long would the peptide be that is translated from this MILNA sequence: 5-AUGGGCUACCGA-3? a. 0 b. 2 c. 3 d. 4
Figure 37.5 Heat shock proteins (HSP) are so named because they help refold misfolded proteins. In response to increased temperature (a “heat shock"), heat shock proteins are activated by release from the NR/HSP complex. At the same time, transcription of HSP genes is activated. Why do you think the cell responds to a heat shock by increasing the activity of proteins that help refold misfolded proteins?
The Adaptive Immune Response Is a Specific Defense Against Infection Researchers have been having a difficult time developing a vaccine against a certain pathogenic virus as a result of the lack of a weakened strain. They turn to you because of your wide knowledge of recombinant DNA technology and the immune system. How could you vaccinate someone against the virus, using a cloned gene from the virus that encodes a cell-surface protein?
Some cancer cells are insensitive to typical chemotherapy. Research into the mechanisms underlying this insensitivity uncovered an ability by these cells to pump the treatment drug out of the cell against its concentration gradient. Additional drugs have been developed that inhibit the pump, thus trapping the chemotherapeutic agent inside to promote cancer cell destruction. The Figure shows what happens when two types of cells are treated with a 3H-labeled anti-cancer drug, paclitaxel. Which set of cells (A or B) would be described as resistant to the cancer treatment? Explain your answer. What type of transport are the resistant cells using?
Biologists have long been interested in the effects of radiation on cells. In one experiment, researchers examined the effect of radium on mitosis of chick embryo cells growing in culture. A population of experimental cells was examined under the microscope for the number of cells in telophase (as a measure of mitosis occurring) before, during, and after exposure to radium. The results are shown in the Figure. What is the effect of radium exposure on mitosis? Source: R. G. Canti and M. Donaldson. 1926. The effect of radium on mitosis in vitro. Proceedings of the Royal Society of London, Series B, Containing Papers of a Biological Character 100:413419.

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