If a protein that contains one peptide chain has no cysteine residues, which step(s) in the chemical strategy for determining primary structure is NOTrequired?O Cleavage of intrachain disulfide bondsO Alternate fragmentation and sequencing to generate overlapping sequencesO Construction of the overall protein sequenceO Terminal residue identificationO Separation and purification of peptide chainsO Peptide chain fragmentation and sequence determinationO Pinpointing the location of disulfide bonds

Answered: If a protein that contains one peptide…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

If a protein that contains one peptide chain has no cysteine residues, which step(s) in the chemical strategy for determining primary structure is NOT
required?
O Cleavage of intrachain disulfide bonds
O Alternate fragmentation and sequencing to generate overlapping sequences
O Construction of the overall protein sequence
O Terminal residue identification
O Separation and purification of peptide chains
O Peptide chain fragmentation and sequence determination
O Pinpointing the location of disulfide bonds

Expert Solution

Step 1

Peptides are formed by the joining of the individual amino acids via the condensation reaction, which involves the removal of water. The end of the protein with a free amino group is called N-terminus, whereas the end of the protein with a free carboxyl group is called the C-terminus.
In the proteins, the primary structure consists of the sequence of the protein. The primary structure can be identified by employing different chemicals.