A peptide has the sequenceGlu-His-Trp-Ser-Gly-Leu-Arg-Pro-GlyThe pK₁ values for the peptide's side chains, terminal amino groups, and carboxyl groups are provided in the table.Amino acid Amino pKa Carboxyl pKa Side-chain pKaglutamate9.602.344.25histidine9.171.826.009.392.389.152.219.602.349.602.369.042.1710.961.99tryptophanserineglycineleucinearginineprolineCalculate the net charge of the molecule at pH 3.Calculate the net charge of the molecule at pH 8.12.48net charge at pH 3:net charge at pH 8: Calculate the net charge of the molecule at pH 11.Estimate the isoelectric point (pl) for this peptide.net charge at pH 11:pl:

Recall that:Amino acid sequences are written with N-terminal amino acid on the left and C-terminal…

Answered: A peptide has the sequence…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Identify the 20 amino acids and their corresponding three-letter and one-letter abbreviations
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At what pH can you separate the following peptides: ala-ala-ala, glu-glu-glu, his-his-his? OA 2.0 B. 4.0 C. 5.0 O D. 7.0 E. 9.5
1) Indicate the total charge of the peptide at pH 7.0, 9.0, and 11.0 2) Determine the isoelectric point (pI) for this peptide. Show the relevant pKa’s and your calculation. 3) What is the fraction of the peptide that has the N-terminal amino group deprotonated at pH 9.
Suppose you have a solution of a protein, which contains a specific Tyr residue that has an actual (measured) pKa of 8.8. The protein binds a ligand by several noncovalent interactions, one of which is a hydrogen bond in which the Tyr phenolic hydroxyl group must serve as a hydrogen bond donor. Calculate the percentage of the protein molecules in which that tyrosyl residue's phenolic hydroxyl group could serve as a hydrogen bond donor at pH 8.5
You have a peptide that has the following amino acid sequence: GPMG Draw the structure of the polypeptide's most protonated form, and its charge. Then draw the structures of the increasingly deprotonated forms, along with their charges. Use the information from these structures to calculate the pl (isoelectric point) of the peptide using the pKa values provided in the table below. Write the pl as x.yz, for example, 7.62 or 3.09. Group Terminal a carboxyl group Aspartic acid Glutamic acid Histidine Terminal a-amino group Cysteine Lysine Tyrosine Arginine Acid EM 2-0" + H -N H H N-H H N-H T Base 1 1. تر H -5 H H O™ N-H Typical pK, 3.1 4.1 6.0 8.0 8.3 10.8 10.9 12.5
Consider each dipeptide. Part: 0 / 4 Part 1 of 4 HO || H H H3N C-C-N-C-COO Identify the amino acid(s) present in this structure. Check all that apply. U U U glycine cysteine valine alanine Ophenylalanine ☐ U U asparagine tyrosine threonine leucine glutamine serine ☐ isoleucine methionine None of the above X
Provide the correct three-letter abbreviation for the following amino acid: Gln ← H₂N-CH-C-C 1 CH3 Ala Arg Asn Asp Cys Glu Gly His lle Leu Lys 包

Refer to following peptide structure: H&N CH3 IZ CH3- CH₂OH IZ OH ZI CH3 CH3. OH IZ OH IZ CH2 CONH2 parce 0 IZ Calculate the net charge of this peptide at pH = 12. OH CH3 What is the identity of the second residue in the peptide shown below? Enter the capitalized single letter abbreviation. 0- Estimate the pl and round to the nearest whole number. Use pKa values provided in lecture.

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