You have a peptide that has the following amino acid sequence: GPMG Draw the structure of the polypeptide's most protonated form, and its charge. Then draw the structures of the increasingly deprotonated forms, along with their charges. Use the information from these structures to calculate the pl (isoelectric point) of the peptide using the pKa values provided in the table below. Write the pl as x.yz, for example, 7.62 or 3.09. Group Terminal a carboxyl group Aspartic acid Glutamic acid Histidine Terminal a-amino group Cysteine Lysine Tyrosine Arginine Acid EM 2-0" + H -N H H N-H H N-H T Base 1 1. تر H -5 H H O™ N-H Typical pK, 3.1 4.1 6.0 8.0 8.3 10.8 10.9 12.5
You have a peptide that has the following amino acid sequence: GPMG Draw the structure of the polypeptide's most protonated form, and its charge. Then draw the structures of the increasingly deprotonated forms, along with their charges. Use the information from these structures to calculate the pl (isoelectric point) of the peptide using the pKa values provided in the table below. Write the pl as x.yz, for example, 7.62 or 3.09. Group Terminal a carboxyl group Aspartic acid Glutamic acid Histidine Terminal a-amino group Cysteine Lysine Tyrosine Arginine Acid EM 2-0" + H -N H H N-H H N-H T Base 1 1. تر H -5 H H O™ N-H Typical pK, 3.1 4.1 6.0 8.0 8.3 10.8 10.9 12.5
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:You have a peptide that has the following amino acid sequence:
GPMG
Draw the structure of the polypeptide's most protonated form, and its charge. Then draw
the structures of the increasingly deprotonated forms, along with their charges.
Use the information from these structures to calculate the pl (isoelectric point) of the
peptide using the pKa values provided in the table below.
Write the pl as x.yz, for example, 7.62 or 3.09.
Group
Terminal a carboxyl
group
Aspartic acid
Glutamic acid
Histidine
Terminal a-amino group
Cysteine
Lysine
Tyrosine
Arginine
Acid
EM
2-0"
+ H
-N
H
H N-H
H
N-H
T
Base
1
1.
تر
H
-5
H
H
O™
N-H
Typical pK,
3.1
4.1
6.0
8.0
8.3
10.8
10.9
12.5
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