Hydrolysis of β-endorphin (a peptide containing 31 amino acid residues) produces the following amino acids: Tyr 1), Gly 3), Phe 2), Met, Thr 3), Ser 2), Lys 5), Gin 2), Pro, Leu 2), Val 2), Asn 2), Ala 2), lle, His, and Glu Treatment with carboxypeptidase liberates Gln. Treatment with DNFB liberates DNP-Tyr. Treatment with trypsin produces the following peptides: Lys, Gly—Gln, Asn—Ala—He—Val—Lys, Tyr—Gly—Gly—Phe—Met—Thr—Ser—Glu—Lys, Asn—Ala—His—Lys, Ser—Gln—Thr—Pro—Leu— Val—Thr—Leu—Phe—Lys Treatment with chymotrypsin produces the following peptides: Lys—Asn—Ala—He—Val—Lys—Asn—Ala—His—Lys—Lys—Gly—Gln Tyr—Gly—Gly—Phe Met—Thr—Ser—Glu—Lys—Ser—Gin—Thr—Pro—Leu—Val—Thr—Leu—Phe What is the primary sequence of β-endorphin?
Hydrolysis of β-endorphin (a peptide containing 31 amino acid residues) produces the following amino acids: Tyr 1), Gly 3), Phe 2), Met, Thr 3), Ser 2), Lys 5), Gin 2), Pro, Leu 2), Val 2), Asn 2), Ala 2), lle, His, and Glu Treatment with carboxypeptidase liberates Gln. Treatment with DNFB liberates DNP-Tyr. Treatment with trypsin produces the following peptides: Lys, Gly—Gln, Asn—Ala—He—Val—Lys, Tyr—Gly—Gly—Phe—Met—Thr—Ser—Glu—Lys, Asn—Ala—His—Lys, Ser—Gln—Thr—Pro—Leu— Val—Thr—Leu—Phe—Lys Treatment with chymotrypsin produces the following peptides: Lys—Asn—Ala—He—Val—Lys—Asn—Ala—His—Lys—Lys—Gly—Gln Tyr—Gly—Gly—Phe Met—Thr—Ser—Glu—Lys—Ser—Gin—Thr—Pro—Leu—Val—Thr—Leu—Phe What is the primary sequence of β-endorphin?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Hydrolysis of β-endorphin (a peptide containing 31 amino acid residues) produces the following
amino acids:
Tyr 1), Gly 3), Phe 2), Met, Thr 3), Ser 2), Lys 5), Gin 2), Pro, Leu 2), Val 2), Asn 2), Ala 2), lle, His, and Glu
Treatment with carboxypeptidase liberates Gln. Treatment with DNFB liberates DNP-Tyr. Treatment with trypsin produces the following peptides:
Lys, Gly—Gln, Asn—Ala—He—Val—Lys, Tyr—Gly—Gly—Phe—Met—Thr—Ser—Glu—Lys, Asn—Ala—His—Lys, Ser—Gln—Thr—Pro—Leu— Val—Thr—Leu—Phe—Lys
Treatment with chymotrypsin produces the following peptides:
Lys—Asn—Ala—He—Val—Lys—Asn—Ala—His—Lys—Lys—Gly—Gln
Tyr—Gly—Gly—Phe
Met—Thr—Ser—Glu—Lys—Ser—Gin—Thr—Pro—Leu—Val—Thr—Leu—Phe
What is the primary sequence of β-endorphin?
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