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How many DNA fragments were produced by the enzyme Ava II?
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Solved in 2 steps
- Is there any situation in which DNA is made based on a RNA template? What is the enzyme involved?The BamH1 enzyme comes at a concentration of 100,000 U/ml. You are asked to digest 20 ug of DNA with this enzyme. Determine: a) How many units will you need? b) How will you dispense them?a) Explain how oxidation of a substrate proceeds without oxygen. (b) Describe what is meant by the antiparallel arrangement of DNA.
- Why is it not surprising that the addition of nucleotides to a growing DNA chain takes place by nucleophilic substitution?Which statement is TRUE regarding the DNA ligase mechanism? A)the last step of the reaction proceeds through a tetrahedral intermediate B)ATP is an obligate donor of an adenylyl group in the reaction of the bacterial enzyme C)The high energy of a phosphoanhydride bond is conserved in the reaction D)The phosphate of the AMP product is linked to the 3'-OH of the ribose E)ATP is required as an energy source to overcome the transition state barrierWhat is the name of the enzyme that add nucleotides to a growing DNA strand? What kind of bonds does this enzyme form between the nucleotides?
- What is the importance of proteins for living beings? What is the name of the DNA duplication process? What is the main enzyme that participates in it?An archaeon (Sulfolobus acidocaldarius) found in acidic hot springs contains a topoisomerase that catalyzes the ATP-driven introduction of positive supercoils into DNA. How might this enzyme be advantageous to this unusual organism?Name the enzymes that cut the DNA at specific sequences and create sticky ends.
