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How many DNA fragments were produced by the enzyme Ava II?
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- The BamH1 enzyme comes at a concentration of 100,000 U/ml. You are asked to digest 20 ug of DNA with this enzyme. Determine: a) How many units will you need? b) How will you dispense them?a) Explain how oxidation of a substrate proceeds without oxygen. (b) Describe what is meant by the antiparallel arrangement of DNA.Chlorambucil is a nitrogen mustard. What is the structure of its covalent adduct to DNA?
- Which statement is TRUE regarding the DNA ligase mechanism? A)the last step of the reaction proceeds through a tetrahedral intermediate B)ATP is an obligate donor of an adenylyl group in the reaction of the bacterial enzyme C)The high energy of a phosphoanhydride bond is conserved in the reaction D)The phosphate of the AMP product is linked to the 3'-OH of the ribose E)ATP is required as an energy source to overcome the transition state barrierI read that vinyl chloride exposure is associated with an increased risk of a rare form of liver cancer (hepatic angiosarcoma), as well as brain and lung cancers, lymphoma, and leukemia. But my question is what gene(s) are being mutated by this type of taxic gas?An archaeon (Sulfolobus acidocaldarius) found in acidic hot springs contains a topoisomerase that catalyzes the ATP-driven introduction of positive supercoils into DNA. How might this enzyme be advantageous to this unusual organism?
