How does the predicted molecular weight of AlaE (16949.07) compare with the molecular weight of the unmodified protein in the Western Blot attached? Are they consistent? kDa55 Cysless352010F16CE39CL860G125C++++P←UM18CV51C+ +Y96C+F139C++++M36CK72C-+MTSES+ NEM+++mPEG5K+P|D106C+UR143C+P-+MTSES+- NEM+++mPEG5K◆U+P+U+ MTSES+ - NEM+++mPEG5K+PTU- + MTSES- NEM+++mPEG5K+++Fig. 1 SCAM analysis of AlaE. Western blot analysis of whole celllysates from cells harbouring plasmids expressing cysteine-free('Cysless') or single cysteine variants of alaE with and without treatmentwith NEM, MTSES and mPEG5K. PEGylated (P) and unmodified proteinare indicated with arrows.

SCAM (Substituted Cysteine Accessibility Method) is an analytical technique which is used to study…

Answered: How does the predicted molecular weight…

How does the predicted molecular weight of AlaE (16949.07) compare with the molecular weight of the unmodified protein in the Western Blot attached? Are they consistent?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

How does the predicted molecular weight of AlaE (16949.07) compare with the molecular weight of the unmodified protein in the Western Blot attached? Are they consistent?

kDa
55 Cysless
35
20
10
F16C
E39C
L860
G125C
+++
+P
←U
M18C
V51C
+ +
Y96C
+
F139C
+
+++
M36C
K72C
-+MTSES
+ NEM
+++mPEG5K
+P
|D106C
+U
R143C
+P
-+MTSES
+- NEM
+++mPEG5K
◆U
+P
+U
+ MTSES
+ - NEM
+++mPEG5K
+P
TU
- + MTSES
- NEM
+++mPEG5K
+++
Fig. 1 SCAM analysis of AlaE. Western blot analysis of whole cell
lysates from cells harbouring plasmids expressing cysteine-free
('Cysless') or single cysteine variants of alaE with and without treatment
with NEM, MTSES and mPEG5K. PEGylated (P) and unmodified protein
are indicated with arrows.

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