How can we visualize the effect of supercoiling on the structure of DNA? (describe supercoiling)

Q: Draw the complete structure of the trinucleotide 5'-A-G-C-3' (DNA).

A: These nucleotides are connected by phosphodiester bonds between the 3' carbon of one sugar molecule…

Q: In DNA purification, which step facilitates the separation of DNA from RNA? Also, what is the…

A: We have to learn how to clean DNA and split it from RNA. The question is asking which part of the…

Q: What is the physical or structural difference between heterochromatin (also called "heterochromatic…

A: The term "chromatin" describes the DNA and protein combination that makes up the chromosomes present…

Q: 51. In 1973, Cohen and Boyer created the first chimera by successfully incorporating the frog rRNA…

A: In 1973, Boyer and Cohen successfully recombined the two plasmids pSC 101 that contain gene…

Q: You start with a relaxed DNA plasmid that is 1000bp in length. a). What is the starting linking…

A: Since you have posted a question with multiple sub-parts, we will solve the first sub-part for you.…

Q: Although DNA polymerases require both a template and a primer, the following single-stranded…

A: DNA polymerases require both a template and a primer to synthesize DNA. However, some…

Q: In humans, the synthesis of Okazaki fragments requires:       DNA polymerase III and DNA polymerase…

A: Okazaki fragments are short, newly synthesized DNA fragments formed on the lagging strand during DNA…

Q: Questions are talking about amplification of DNA When we run the gel, we also run a ladder why?

A: A DNA ladder is the mixture of amplified DNA fragments of different size range which is used as…

Q: What is the relationship between the free energy of supercoiling and the superhelical density? How…

A: Deoxyribonucleic acid (DNA) is a compound made up of two nucleotide bases which wrap around together…

Q: During agarose gel electrophoresis, why does DNA move through the gel when electric current is…

A: Gel electrophoresis is basically a method that aid in separating DNA fragments and other molecules…

Q: BamHI Haell Both 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb Figure 2 (i) How long is the original DNA molecule?…

A: Restriction digestion is a process in which DNA is cut at specific sites,

Q: A geneticist is interested in determining the locations of methylated cytosines within a fragment of…

A: Sodium bisulfite (NaHSO3) is a potent deamination agent. It removes an amino group (-NH2) from…

Q: 11) Examine the following two DNA sequences. Sequence 1: ATGCGATGCTAGCAT Sequence 2: ATGCGATGATAGCAT…

A: Introduction :- Large, intricate molecules known as proteins play a variety of vital functions in…

Q: 25. The restriction enzymes Kpnl and Acc651 recognize and cleave the same 6-bp sequence. You have a…

A: Restriction enzymes are molecular scissors that are used in recombinant DNA technology to cut DNA at…

Q: What is the role of GelRed® in Agarose gel electrophoresis of DNA fragments?

A: Electrophoresis is the technique which help in separating the RNA,  DNA and protein on the basis of…