Genomic sequencing cannot be used to: Predict a protein coding gene Predict structure and function of a protein encoded in a gene Locate similar sequences (sequence homology) Locate repetitive sequences Predicting alternative splicing patterns
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- b) A gene was isolated from mouse and sequenced by a research group. When the sequencing result was obtained, the research leader requested her assistant to generate the results below (Figure 1) using a Bioinformatics tool. >>> Descriptions Graphic Summary Sequences producing significant alignments select all 200 sequences selected Alignments Taxonomy Mus musculus itin 1(Sit1), transcript variant 1. mRNA Mus musculus Siapha(S)RNA completed Mus musculus Sic2alpha orain IS2alphai mRNA complete ods PREDICTED: Mus.caroll situin 1(Sirt1) transcript variant X1. mRNA ✔Mus musculus situin 1(S1), transcript variant 2.mRNA ✔PREDICTED: Anvicanthis niloticus situin 1 (Sirt1) transcript variant X1 mRNA PREDICTED: Acanthis nicticus siruin 1.(Sirt1) transvaatX2 NA (i) Description (ii) Download ✔Mus musculus mutent stem cell CRL-2070 NE cDNA. RIKEN M-length enriched library, clone:G430107915 productsituin 10sent: 0030 6805 95% PREDICTED:Mastommys.coucha sittuin 1 (Sirt1), transcript variant X1. mRNA…Explain the steps of RNA-Sequencing down below, and explain the steps of anylazing the data.In a genome project, the following genomic DNA sequences were obtained. Assemble the sequences into a contig. Using the assembled sequence, perform a BLASTn search. Does the search produce sequences similar to your assembled sequence? 5’ TCGGGGTCCTGGGATCTCATCACTGCAGCGC 3’ 5’ACTGCAGCGCTTTCCCAGCGGGCGGTGGTAC 3’ 5’GGGCGGTGGTACTCGGGAAGTCAGGAGTGTT 3’ 5’AGGAGTGTTTAAAACCTGGGGACTGGTTTTG 3’ 5’TGGTTTTGGGGGCGCTGAAGGCAGCGCAGGA 3’
- You have assembled a new prokaryotic genome and are annotating the genes. If you were to search for highly conserved sequences necessary for normal gene expression, what type of annotation are you doing? Group of answer choices ad hoc annotation homology based annotation preliminary annotation ab initio annotationWhat are the similarities and/or differences between interpreting Multiple Sequence Alignment (MSA) of conserved sequences at the DNA level versus the Amino Acid level? Please site references if possible and have 5+ sentences.Place the steps necessary to perform RNA-Seq in the correct order. Drag the text blocks below into their correct order. Reset MAAAAAAAAAKK MAAAAAAAAAAM Compare sequences to known genome sequence. Create cDNA using reverse transcription with primers complementary to linkers. Attach linkers to the ends of the RNAs. Perform next-generation DNA sequencing. Isolate RNA from cells or tissues of interest. Fragment the RNAs.
- Explain how a multiple sequence alignment can identify functional sites in a genetic sequence.This descriptor refers to the highest alignment score of all matched sequences to the sequence placed in the BLASTn search. Query cover Maximum score and Total score E-value Ident Accession numberWhat is meant by the term "alignment" in genomic research and in what ways does alignment aid sequence assembly? How can relying on alignment lead to potential errors?
- A Sanger product of the sequencing of a template DNA is presented below. It is the polymorphic peptide sequence of Protein Ser-Gly-Lys-Glu-Gly-Lys-Lys. Determine the sequence of template DNA and the mRNA sequence from this template DNA. Briefly explain.What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.A protein has the following amino acid sequence: Met-Tyr-Asn-Val-Arg-Val-Tyr-Lys-Ala-Lys-Trp-Leu-Ile-His-Thr-Pro You wish to make a set of probes to screen a cDNA library for the sequence that encodes this protein. Your probes should be at least 18 nucleotides in length. Q. How many different probes must be synthesized to be certain that you will find the cDNA sequence that specifies the protein?