For an enzymatic reaction, the following data were obtained for two different initial enzyme concentrations: [S](g/L) v ([E0] = 0,015 g/L)(g/L.min) v ([E0] = 0,00875 g/L) (g/L.min) 40 2,28 1,34 20 1,74 1,02 13,4 1,4 0,82 10 1,18 0,68 8 1 0,58 (a)Calculate Vmax (in g/(L.min)) for the initial enzyme concentration equal to 0.015 g/L. (b)Calculate Vmax (in g/(L.min)) for the initial enzyme concentration equal to 0.00875 g/L. (c)Calculate Km (in g/L) for the initial enzyme concentration equal to 0.015 g/L. (d)Calculate Km (in g/L) for the initial enzyme concentration equal to 0.00875 g/L.
For an enzymatic reaction, the following data were obtained for two different initial enzyme concentrations: [S](g/L) v ([E0] = 0,015 g/L)(g/L.min) v ([E0] = 0,00875 g/L) (g/L.min) 40 2,28 1,34 20 1,74 1,02 13,4 1,4 0,82 10 1,18 0,68 8 1 0,58 (a)Calculate Vmax (in g/(L.min)) for the initial enzyme concentration equal to 0.015 g/L. (b)Calculate Vmax (in g/(L.min)) for the initial enzyme concentration equal to 0.00875 g/L. (c)Calculate Km (in g/L) for the initial enzyme concentration equal to 0.015 g/L. (d)Calculate Km (in g/L) for the initial enzyme concentration equal to 0.00875 g/L.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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For an enzymatic reaction, the following data were obtained for two different initial enzyme concentrations:
| [S](g/L) | v ([E0] = 0,015 g/L)(g/L.min) | v ([E0] = 0,00875 g/L) (g/L.min) |
|
40 |
2,28 |
1,34 |
|
20 |
1,74 |
1,02 |
|
13,4 |
1,4 |
0,82 |
|
10 |
1,18 |
0,68 |
|
8 |
1 |
0,58 |
(a)Calculate Vmax (in g/(L.min)) for the initial enzyme concentration equal to 0.015 g/L.
(b)Calculate Vmax (in g/(L.min)) for the initial enzyme concentration equal to 0.00875 g/L.
(c)Calculate Km (in g/L) for the initial enzyme concentration equal to 0.015 g/L.
(d)Calculate Km (in g/L) for the initial enzyme concentration equal to 0.00875 g/L.
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