Following 'salting out' experiments with crude samples, you now have 5 proteins, named after your friends, in your sample. You want to further purify your proteins in solution (pH 7.6). Their characteristics are given in the table below. Protein names Annie Molecular weight (Da) 46,000 11,500 27,500 31,000 200,000 pl 6.2 Bob 10.4 Chris Dan 6.9 4.5 Emily 8.6 If you separate this sample by SDS-PAGE (the sample was loaded at the top), which of the following proteins would you expect to find at the bottom of the gel? A. Annie B. Bob O C. Chris O D. Dan O E. Emily
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
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