Following data were obtained in a study of enzymatic reaction that follows the MichaelisMenten kinetics. Estimate the Km for this enzyme.of substrate (mm)Concentration51015201000A. Km = 5 mMmmmmmB. Km = 10 mMC. Km 15 mM=D. Km = 20 mME. Km 1000 mMVo (μmol/min)160300432655822 Which of the following about phosphorylation of glucose is CORRECT?This prevents leakage of the molecule out of the cellI.II.This allows specific binding to the active site of the enzymesThis forces all the glucose to go to glycolysisIII.IV.This allows substrate level phosphorylation to synthesis ATPA. I and IIB. I and IIIC. I, II and IIID. II, III and IVE. All of them

Enzymes kinetics - is the study of the reaction rate of enzyme catalyzed reactions. The reaction…

Answered: Following data were obtained in a study…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Following data were obtained in a study of enzymatic reaction that follows the Michaelis Menten kinetics. Estimate the Km for this enzyme. Concentration of substrate (mm) 5 10 15 20 1000 A. Km 5 mM B. Km 10 mM = C. Km 15 mM D. Km = 20 mM E. Km 1000 mM Vo (μmol/min) 160 300 432 655 822