Explain shortly how the chromatography will be influenced in a C18 reverse phase liquid chromatography column when: (motivate your answer) a) a 100 meter column with 10 000 theoretical plates is used in comparison to a 30 meter column with 30 000 theoretical plates. b) the flow rate is decreased. c) the temperature of the mobile phase is increased. d) the particle size of the stationary material is decreased. e) the particle shape of the stationary material is made more uneven. f) decreasing the column temperature.
Explain shortly how the chromatography will be influenced in a C18 reverse phase liquid chromatography column when: (motivate your answer) a) a 100 meter column with 10 000 theoretical plates is used in comparison to a 30 meter column with 30 000 theoretical plates. b) the flow rate is decreased. c) the temperature of the mobile phase is increased. d) the particle size of the stationary material is decreased. e) the particle shape of the stationary material is made more uneven. f) decreasing the column temperature.
Chemistry
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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Explain shortly how the chromatography will be influenced in a C18 reverse phase liquid chromatography column when:
(motivate your answer)
a) a 100 meter column with 10 000 theoretical plates is used in comparison to a 30 meter column with 30 000 theoretical plates.
b) the flow rate is decreased.
c) the temperature of the mobile phase is increased.
d) the particle size of the stationary material is decreased.
e) the particle shape of the stationary material is made more uneven.
f) decreasing the column temperature.
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