In Chromatography - Equilibrium Binding Isotherms, If diffusion and dispersion are negligible a) Explain why the effective velocity is a constant if the binding of the separand to the matrix follows a linear isotherm. b) In analytical chromatography, the band or elution peak is very narrow and has a symmetrical Gaussian shape. Explain why

In Chromatography - Equilibrium Binding Isotherms, If diffusion and dispersion are negligible a) Explain why the effective velocity is a constant if the binding of the separand to the matrix follows a linear isotherm. b) In analytical chromatography, the band or elution peak is very narrow and has a symmetrical Gaussian shape. Explain why

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

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Question

In Chromatography - Equilibrium Binding
Isotherms,
If diffusion and dispersion are negligible
a) Explain why the effective velocity is a
constant if the binding of the separand to the
matrix
follows a linear isotherm.
b) In analytical chromatography, the band or
elution peak is very narrow and has a
symmetrical
Gaussian shape. Explain why
c) Explain qualitatively why the front end of
the separand band always self-sharpens and
the
trailing end tends to spread out if the binding
follows a Langmuir isotherm.
d) What would happen to the elution
peak/migration band if the column is
"overloaded" (c > S).

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