In Chromatography - Equilibrium Binding Isotherms, If diffusion and dispersion are negligible a) Explain why the effective velocity is a constant if the binding of the separand to the matrix follows a linear isotherm. b) In analytical chromatography, the band or elution peak is very narrow and has a symmetrical Gaussian shape. Explain why
In Chromatography - Equilibrium Binding Isotherms, If diffusion and dispersion are negligible a) Explain why the effective velocity is a constant if the binding of the separand to the matrix follows a linear isotherm. b) In analytical chromatography, the band or elution peak is very narrow and has a symmetrical Gaussian shape. Explain why
Chapter93: Refractometry
Section: Chapter Questions
Problem 1P
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![In Chromatography - Equilibrium Binding
Isotherms,
If diffusion and dispersion are negligible
a) Explain why the effective velocity is a
constant if the binding of the separand to the
matrix
follows a linear isotherm.
b) In analytical chromatography, the band or
elution peak is very narrow and has a
symmetrical
Gaussian shape. Explain why
c) Explain qualitatively why the front end of
the separand band always self-sharpens and
the
trailing end tends to spread out if the binding
follows a Langmuir isotherm.
d) What would happen to the elution
peak/migration band if the column is
"overloaded" (c > S).](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fb2895e27-4fbe-4585-a783-1a45d44d6750%2Fffd7481c-d742-47a7-9611-6da4868b3831%2Fsy1mmh_processed.jpeg&w=3840&q=75)
Transcribed Image Text:In Chromatography - Equilibrium Binding
Isotherms,
If diffusion and dispersion are negligible
a) Explain why the effective velocity is a
constant if the binding of the separand to the
matrix
follows a linear isotherm.
b) In analytical chromatography, the band or
elution peak is very narrow and has a
symmetrical
Gaussian shape. Explain why
c) Explain qualitatively why the front end of
the separand band always self-sharpens and
the
trailing end tends to spread out if the binding
follows a Langmuir isotherm.
d) What would happen to the elution
peak/migration band if the column is
"overloaded" (c > S).
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