None How will you quantify the unknown sample in gaschromatography analysis? Re-arrange the steps in order.StepChoose...StepStepIStepStep 1Choose the internal standard (is)Find the Relative response (Rf) of a component (X) to theinternal standard (is) Find the Response factor Rf using RfArea / Concentration Find the concentration of X using Rf',concentration and area of is IS at a constant concentration isadded to all standards

To quantify an unknown sample using gas chromatography, you typically follow a specific set of steps…

Answered: How will you quantify the unknown…

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter4: Least-squares And Calibration Methods

Section: Chapter Questions

Problem 10P

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Qno1: why there is a need of chromatography and chromatography thecniqs. B) when and where we should use normal and reverse phase chromatography
Use the following information collected from a SIZE-EXCLUSION CHROMATOGRAPHY experiment to answer the questions below. MW Standards Molecular Weight (kDa) Elution Volume (mL) blue dextran 1000 85 myoglobin 17.2 200 TIM 53.3 178 hemoglobin 62.2 156 IgG 140 122 ATCase 307.9 94 Prepare a graph showing the linear relationship between log function of molecular weight against Ve/Vo. The R2value must be 0.9 or higher. What is the linear equation from the standards given? What is the R2value?
anch of the following components cannot be detected by gas chromatography? 4 Essential oils Alcohols b) Volatile compounds d) Non-volatile compounds 13- is measured as time elapsed from the point of injection to the peak maximum? a)-Column efficiency d)- Delectability c)-Retention time 14- Which of the following can be used to detect the components in gas chromatography a) non-volatile compounds b) volatile compounds c) Salts d) b&c 15-Which of the following methods is often referred to as mechanical separation processes. a)-Gas Chromatography b)-column chromatography c)- Sedimentation 16- For obtaining better resolution in TLC. and then developed with a second solvent at right angles to the first a) Stepwise b) Descending e) Ascending 17- This is defined as transport of electrically charged particles in a direct-current electric field a) ion exchange chromatography b) Electrophoresis c) ultracentrifuge d) exclusion 18-This figure indicates to schematic Diagram of a) Gas chromatography…
Which of the following statements is incorrect? O A. In reversed-phase chromatography, the stationary phase is nonpolar. B. In reversed-phase chromatography, increasing the mobile-phase polarity increases the elution time. C. In reversed-phase chromatography, most polar component is eluted last. D. In normal-phase chromatography, the most polar component is eluted last. E. In normal-phase chromatography, the stationary phase is polar.
This is the graph for gel filtration chromatography. Can you please explain why the graph has the maximum and minimum peak and relate it with the gel filtration concept such as the volume of elution, molecular weight, and so on. Thank you . Graph for Result 1: (Volume of elution (mL) with 1% bromophenol blue) Volume of Elution (mL) vs Corrected OD 1.789 1.8 1.6 1.4 1.2 0.745 0.8 0.477 0.6 0.379.400.452 0.406 0.393 0.254 d28 0.25 0.4 9:128 0.161 0.115 0.2 0.060 2 6. 10 12 14 16 Volume of Elution (mL) Corrected OD
two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What mobile phase condition/composition (LC separation) was used?
Which statement(s) are correct for gas chromatography? A) the components in a mixture can be identified from their retention time. B) the relative peak areas give the proportions of components in a mixture. C) Calibration curves are used to confirms the concentrations of components in a mixture.
Please justify your answer
Three students did a chromatography experiment, where Rf = distance of solute / distance of solvent. What could be the possible errors why student 3 had results that are quite far from that of students A and B?
Answer sections C, D and E in the same answer book. Section D (Chromatography) Question 8 The following data were obtained for the analysis of a mixture of compounds (A-D) by HPLC: Peak Retention time (min) | Width of peak base (min) non-retained 2.1 A 6.4 10.51 B 11.3 1.03 C 13.1 1.25 D 21.6 1.62 a) Using peak C. calculate the number of plates (N) in this column. b) For peak D, calculate the capacity factor (k'). c) For peak B and C, calculate the resolution (R).
Fill in the blanks with either of these words: Polar or nonpolar Reverse-phase chromatography uses a _____________ stationary phase and a _____________ mobile phase. The more _________________ the mobile phase becomes, the stronger the eluent strength.
D10)

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