Experiment: UV-Vis Spectrophotometry and Beer’s Law Absorption of Light of Dyes in Beverages   5 mL of a lemon soft drink was pipette into a 25 mL volumetric flask and diluted to the mark with distilled water. 8 mL of this diluted solution was pipette into a test tube, then 2 mL of distilled water was pipette into the same test tube. Analysis by spectrometry found that the concentration of yellow dye in the test tube solution was 4.7 x 10-5 M. Calculate the concentration of yellow dye in the original soft drink.

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Experiment: UV-Vis Spectrophotometry and Beer’s Law

Absorption of Light of Dyes in Beverages

 

5 mL of a lemon soft drink was pipette into a 25 mL volumetric flask and diluted to the mark with distilled water. 8 mL of this diluted solution was pipette into a test tube, then 2 mL of distilled water was pipette into the same test tube. Analysis by spectrometry found that the concentration of yellow dye in the test tube solution was 4.7 x 10-5 M. Calculate the concentration of yellow dye in the original soft drink.

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