Draw two reaction coordinate diagrams (DG vs reaction coordinate) that compare the uncatalyzed process of lipid flipping (lipid out à lipid in ) from outer to inner leaflet to the flippase-catalyzed process.
Draw two reaction coordinate diagrams (DG vs reaction coordinate) that compare the uncatalyzed process of lipid flipping (lipid out à lipid in ) from outer to inner leaflet to the flippase-catalyzed process.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Draw two reaction coordinate diagrams (DG vs reaction coordinate) that compare the uncatalyzed process
of lipid flipping (lipid out à lipid in ) from outer to inner leaflet to the flippase-catalyzed process.

Transcribed Image Text:ii) Draw two reaction coordinate diagrams (AG vs reaction coordinate) that compare the uncatalyzed process
of lipid flipping (lipidout → lipidin) from outer to inner leaflet to the flippase-catalyzed process. (Note that an
ATPases like flippases enhance both the rates of a reaction (like a typical enzyme) and thermodynamic profile
of a reaction (because they can couple ATP hydrolysis to a particular reaction, adding ATP/ADP+P; in the
reactants/products)
uncatalyzed barrier on the order of 50 kcal/mol
lipid
catalyzed barrier?
+
NH3
ATP
out
ADP + P₁
Flippase
lipid
out
very slow
(t1/2 in days)
fast
(t₁/2 in minutes)
lipidin
lipid in
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