Determine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis. Table 1: Raw data for the Anthrone testGlucoseSample No.(1) Blank2345678910(μL)501002003004005007501000Unknown(µg)1020406080100150200??Distilled H₂O(μL)1000950900800700600500250Anthronereagent (mL)5555555555A620 F:F12345678BB910111213141516171819202122232425262728+ZOO resourcesASample1zoo 111 lec post lab27 100%fx A6202345678910businessresults from f2f biochem labFile Edit View Insert Format Data Tools Extensions Help Last edit was seconds agoBIS A✰ docs.google.com/spreadsheets/d/1puQXKaLr2WRn59fFDzuA6yS-dEISQLT2tS-TTpF8bpQ/edit#gid=0Sheet1zoo 111 lec and lab$ %0.00 123-BConcentration of Glucose (ug)01020406080100150200unknownDefault (Ari... ▼ 10CwikaA620 - R10.0010.1010.0240.03920.3470.580.6260.9491.9962.413scientifico +A620A620 - R20.0010.0790.150.2880.3610.5150.6230.7760.9762.3622.52.01.51.0Standard Curve of A620 and µg of Glucose-A620 -6.57E-03*x + -0.0206 R² = 0.9830.5D0.0083-EA620 - R300.0380.1080.3190.4090.5440.635500.8031.1652.39X100PConcentration of Glucose (ug)FA6200.00066666666670.072666666670.0940.21540.37233333330.54633333330.6280.84266666671.3792.3883333331502001.MTypeChart editorSetupTenummeLinearLine opacityLabel40%Use EquationShow R²+> LegendCount: 22Line color2px> Horizontal axisSum: 666.5394Avg: 35.08102105Min: 0Max: 200Line thicknessa ShareCustomizeCount Numbers: 19XExplore31Ꭷ●+>

Here we are conducting an assay to determine the amount of glucose in an unknown sample. For this,…

Answered: Determine the amount of glucose in the…

Determine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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